ArticlePloS one2017
Reliable reference genes for normalization of gene expression data in tea plants (Camellia sinensis) exposed to metal stresses.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
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- Screening and validation of reference genes of Gynostemma pentaphyllum with different tissues and under different treatment conditions by RT-qPCR analysis.Molecular biology reports · 2025Article
- Development and selection of stably expressed reference genes for expression normalization in Ribes odoratum under drought stress.Scientific reports · 2025Article
- Transcriptome Analysis Reveals Differentially Expressed Genes Involved in Aluminum, Copper and Cadmium Accumulation in Tea 'Qianmei 419' and 'Qianfu 4'.Plants (Basel, Switzerland) · 2023Article
- Selection and validation of reference genes for normalizing qRT‒PCR gene expression studies inFrontiers in plant science · 2023Article
- Identification of suitable reference genes for expression studies in pomegranate under different biotic and abiotic stress conditions.Molecular biology reports · 2021Article
- Reference genes for quantitative real-time PCR normalization of Cenostigma pyramidale roots under salt stress and mycorrhizal association.Genetics and molecular biology · 2021Article
- Regulation of antioxidant enzymes and osmo-protectant molecules by salt and drought responsive genes in Bambusa balcooa.Journal of plant research · 2021Article
- Genome-wide characterization of tea plant (Camellia sinensis) Hsf transcription factor family and role of CsHsfA2 in heat tolerance.BMC plant biology · 2020Article
- JA-Ile-macrolactone 5b Induces Tea Plant (International journal of molecular sciences · 2020Article
- Identification and validation of superior housekeeping gene(s) for qRT-PCR data normalization inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Identification and evaluation of reliable reference genes for quantitative real-time PCR analysis in tea plants under differential biotic stresses.Scientific reports · 2020Article
- Article
- Nanoparticles Affect the Expression Stability of Housekeeping Genes in Plant Cells.Nanotechnology, science and applications · 2020Article
- Selection of reliable reference genes for gene expression analysis in seeds at different developmental stages and across various tissues in Paeonia ostii.Molecular biology reports · 2019Article
- Validation of Reliable Reference Genes for RT-qPCR Studies of Target Gene Expression inFrontiers in microbiology · 2019Article
- Validation of reference genes for gene expression studies in post-harvest leaves of tea plant (PeerJ · 2019Article
- Article
- Reference gene selection for qRT-PCR assays inPeerJ · 2018Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Tea plants [Camellia sinensis (L.) O. Kuntze] are an important leaf-type crop that are widely used for the production of non-alcoholic beverages in the world. Exposure to excessive amounts of heavy metals adversely affects the quality and yield of tea leaves. To analyze the molecular responses of tea plants to heavy metals, a reliable quantification of gene expression is important and of major importance herein is the normalization of the measured expression levels for the target genes. Ideally, stably expressed reference genes should be evaluated in all experimental systems. In this study, 12 candidate reference genes (i.e., 18S rRNA, Actin, CYP, EF-1α, eIF-4α, GAPDH, MON1, PP2AA3, TBP, TIP41, TUA, and UBC) were cloned from tea plants, and the stability of their expression was examined systematically in 60 samples exposed to diverse heavy metals (i.e., manganese, aluminum, copper, iron, and zinc). Three Excel-based algorithms (geNorm, NormFinder, and BestKeeper) were used to evaluate the expression stability of these genes. PP2AA3 and 18S rRNA were the most stably expressed genes, even though their expression profiles exhibited some variability. Moreover, commonly used reference genes (i.e., GAPDH and TBP) were the least appropriate reference genes for most samples. To further validate the suitability of the analyzed reference genes, the expression level of a phytochelatin synthase gene (i.e., CsPCS1) was determined using the putative reference genes for data normalizations. Our results may be beneficial for future studies involving the quantification of relative gene expression levels in tea plants.
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