ArticleAdipocyte2017
Flow cytometric single cell analysis reveals heterogeneity between adipose depots.
Article in Adipocyte, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Label-Free Detection of Lipid Accumulation in Cells Using Magnetic Levitation.Advanced biology · 2025Article
- MarrowCellDLD: a microfluidic method for label-free retrieval of fragile bone marrow-derived cells.Scientific reports · 2023Article
- Adipokines and Bacterial Metabolites: A Pivotal Molecular Bridge Linking Obesity and Gut Microbiota Dysbiosis to Target.Biomolecules · 2023Review
- Exploring the heterogeneity of white adipose tissue in mouse and man.Current opinion in genetics & development · 2023Review
- Anthocyanins fromMolecules (Basel, Switzerland) · 2021Article
- Mass-Added Density Modulation for Sorting Cells Based on Differential Surface Protein Levels.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
- Exploring the utility of Deep Red Anthraquinone 5 for digital staining of ex vivo confocal micrographs of optically sectioned skin.Journal of biophotonics · 2021Article
- Refining the adipose progenitor cell landscape in healthy and obese visceral adipose tissue using single-cell gene expression profiling.Life science alliance · 2019Article
- Article
- Adipose Tissue Dysfunction as Determinant of Obesity-Associated Metabolic Complications.International journal of molecular sciences · 2019Review
- NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes.Biochemical and biophysical research communications · 2018Article
- Flow Cytometry of Mouse and Human Adipocytes for the Analysis of Browning and Cellular Heterogeneity.Cell reports · 2018Article
- Bone Marrow Adipocyte: An Intimate Partner With Tumor Cells in Bone Metastasis.Frontiers in endocrinology · 2018Review
- Single Cell Oxygen Mapping (SCOM) by Scanning Electrochemical Microscopy Uncovers Heterogeneous Intracellular Oxygen Consumption.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding adipose tissue heterogeneity is hindered by the paucity of methods to analyze mature adipocytes at the single cell level. Here, we report a system for analyzing live adipocytes from different adipose depots in the adult mouse. Single cell suspensions of buoyant adipocytes were separated from the stromal vascular fraction and analyzed by flow cytometry. Compared to other lipophilic dyes, Nile Red uptake effectively distinguished adipocyte populations. Nile Red fluorescence increased with adipocyte size and granularity and could be combined with MitoTracker® Deep Red or fluorescent antibody labeling to further dissect adipose populations. Epicardial adipocytes exhibited the least mitochondrial membrane depolarization and highest fatty-acid translocase CD36 surface expression. In contrast, brown adipocytes showed low surface CD36 expression. Pregnancy resulted in reduced mitochondrial membrane depolarisation and increased CD36 surface expression in brown and epicardial adipocyte populations respectively. Our protocol revealed unreported heterogeneity between adipose depots and highlights the utility of flow cytometry for screening adipocytes at the single cell level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.