ArticleInternational journal of oral science2017
Biofilm three-dimensional architecture influences in situ pH distribution pattern on the human enamel surface.
Article in International journal of oral science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 88 citations in OpenAlex.
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- [The Oral Microbiome: Maintenance of Homeostasis, Disease Associations, and Mechanisms of Pathogenesis].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2026Review
- Role of sucrose-dependent exopolysaccharides in the biofilm development ofApplied and environmental microbiology · 2026Article
- Targeted Photodynamic Therapy for Multispecies Dental Biofilms Using Liposome Encapsulated p-Toluquinone.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2026Article
- Mechanisms of caries induced by sugars: a narratives review from microbial metabolism to oral ecological imbalance and public health strategies for caries prevention.Frontiers in cellular and infection microbiology · 2026Review
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- Potential roles and mechanisms of bacterial peptidylarginine deiminase in dental biofilm mediated byJournal of oral microbiology · 2025Review
- The Effect of Bovine Trypsin on the Adhesion and pH of Dental Plaque Biofilms: An In Vitro Study.International dental journal · 2024Article
- Antibiotic Resistant Biofilms and the Quest for Novel Therapeutic Strategies.Indian journal of microbiology · 2024Review
- Biofilm architecture and dynamics of the oral ecosystem.Biotechnologia · 2024Review
- Article
- Selenomonas sputigena acts as a pathobiont mediating spatial structure and biofilm virulence in early childhood caries.Nature communications · 2023Article
- Synchrotron X-ray Studies of the Structural and Functional Hierarchies in Mineralised Human Dental Enamel: A State-of-the-Art Review.Dentistry journal · 2023Review
- Effect of ProbioticInternational journal of molecular sciences · 2023Article
- Effect of Nystatin on Candida albicans - Streptococcus mutans duo-species biofilms.Archives of oral biology · 2023Article
- Microfabrication approaches for oral research and clinical dentistry.Frontiers in dental medicine · 2023Review
- Roles ofFrontiers in cellular and infection microbiology · 2023Review
- Interkingdom assemblages in human saliva display group-level surface mobility and disease-promoting emergent functions.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Gradients and consequences of heterogeneity in biofilms.Nature reviews. Microbiology · 2022Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
To investigate how the biofilm three-dimensional (3D) architecture influences in situ pH distribution patterns on the enamel surface. Biofilms were formed on human tooth enamel in the presence of 1% sucrose or 0.5% glucose plus 0.5% fructose. At specific time points, biofilms were exposed to a neutral pH buffer to mimic the buffering of saliva and subsequently pulsed with 1% glucose to induce re-acidification. Simultaneous 3D pH mapping and architecture of intact biofilms was performed using two-photon confocal microscopy. The enamel surface and mineral content characteristics were examined successively via optical profilometry and microradiography analyses. Sucrose-mediated biofilm formation created spatial heterogeneities manifested by complex networks of bacterial clusters (microcolonies). Acidic regions (pH<5.5) were found only in the interior of microcolonies, which impedes rapid neutralization (taking more than 120 min for neutralization). Glucose exposure rapidly re-created the acidic niches, indicating formation of diffusion barriers associated with microcolonies structure. Enamel demineralization (white spots), rougher surface, deeper lesion and more mineral loss appeared to be associated with the localization of these bacterial clusters at the biofilm-enamel interface. Similar 3D architecture was observed in plaque-biofilms formed in vivo in the presence of sucrose. The formation of complex 3D architectures creates spatially heterogeneous acidic microenvironments in close proximity of enamel surface, which might correlate with the localized pattern of the onset of carious lesions (white spot like) on teeth.
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