Evidence map›Paper›PMID 28452085›Full record

ArticleMolecular microbiology2017

Use of chimeric type IV secretion systems to define contributions of outer membrane subassemblies for contact-dependent translocation.

Jay E Gordon, Tiago R D Costa, Roosheel S Patel, Christian Gonzalez-Rivera, Mayukh K Sarkar, Elena V Orlova, Gabriel Waksman, Peter J Christie

Open access · hybridAbstract read
In one paragraph

Article in Molecular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
5.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 51 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Comparative Analysis of T4SS Molecular Architectures.Journal of microbiology and biotechnology · 2023
    Review
  7. Role of the CagY antenna projection inInfection and immunity · 2023
    Article
  8. Ligand-DisplayingbioRxiv : the preprint server for biology · 2023
    Article
  9. Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Review
  20. Endogenous membrane stress induces T6SS activity inProceedings of the National Academy of Sciences of the United States of America · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Jay E GordonDepartment of Microbiology and Molecular Genetics, McGovern Medical School, 6431 Fannin St, Houston, TX, 77030, USA.
Tiago R D CostaInstitute of Structural and Molecular Biology, University College London and Birkbeck, Malet Street, London, WC1E 7HX, UK.
Roosheel S PatelDepartment of Microbiology and Molecular Genetics, McGovern Medical School, 6431 Fannin St, Houston, TX, 77030, USA.
Christian Gonzalez-RiveraDepartment of Microbiology and Molecular Genetics, McGovern Medical School, 6431 Fannin St, Houston, TX, 77030, USA.
Mayukh K SarkarDepartment of Microbiology and Molecular Genetics, McGovern Medical School, 6431 Fannin St, Houston, TX, 77030, USA.
Elena V OrlovaInstitute of Structural and Molecular Biology, University College London and Birkbeck, Malet Street, London, WC1E 7HX, UK.
Gabriel WaksmanInstitute of Structural and Molecular Biology, University College London and Birkbeck, Malet Street, London, WC1E 7HX, UK.
Peter J ChristieDepartment of Microbiology and Molecular Genetics, McGovern Medical School, 6431 Fannin St, Houston, TX, 77030, USA.ORCID http://orcid.org/0000-0002-9799-7067
Institute of Structural and Molecular Biology · GB

Funding

TRANSLOCATION OF DNA ACROSS THE AGROBACTERIUM ENVELOPER01GM048746 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI CHRISTIE, PETER J. · 1998 to 2018
$6.9M
Molecular Basis of Infectious Diseases Training GrantT32AI055449 · NIAID · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI LORENZ, MICHAEL C · 2005 to 2025
$2.6M
Structure-function analyses of bacterial Type IV secretion system subassembliesF32AI114182 · NIAID · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI GONZALEZ-RIVERA, CHRISTIAN · 2014 to 2016
$143k
Medical Research Council MR/K012401/1NIAID NIH HHS F32 AI114182NIAID NIH HHS T32 AI055449NIGMS NIH HHS R01 GM048746Wellcome Trust 098302
6 · The paper itself

Abstract

Recent studies have shown that conjugation systems of Gram-negative bacteria are composed of distinct inner and outer membrane core complexes (IMCs and OMCCs, respectively). Here, we characterized the OMCC by focusing first on a cap domain that forms a channel across the outer membrane. Strikingly, the OMCC caps of the Escherichia coli pKM101 Tra and Agrobacterium tumefaciens VirB/VirD4 systems are completely dispensable for substrate transfer, but required for formation of conjugative pili. The pKM101 OMCC cap and extended pilus also are dispensable for activation of a Pseudomonas aeruginosa type VI secretion system (T6SS). Chimeric conjugation systems composed of the IMC

Indexed as

Agrobacterium tumefaciensBacterial Outer Membrane ProteinsBacterial ProteinsConjugation, GeneticDNA, BacterialDNA-Binding ProteinsEscherichia coliFimbriae, BacterialProtein BindingProtein TransportType IV Secretion SystemsVirulence FactorsBacterial Outer Membrane ProteinsBacterial ProteinsDNA, BacterialDNA-Binding ProteinsType IV Secretion SystemsVirulence Factors

Identifiers

PMID28452085
PMCPMC5518639
OpenAlexW2609329012

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.