Article3 Biotech2017
Assessment of genetic diversity in Costus pictus accessions based on RAPD and ISSR markers.
Article in 3 Biotech, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Genetic Variation of Spongy Moth (Insects · 2026Article
- Unraveling the genetic diversity and population structure of Mesua ferrea L. Through RAPD and ISSR markers for effective conservation.Journal, genetic engineering & biotechnology · 2026Article
- Exploring the Antifungal Activity and Action ofAntibiotics (Basel, Switzerland) · 2022Article
- Assessment of genetic diversity inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- RAPD and ISSR marker assessment of genetic diversity in3 Biotech · 2017Article
- Molecular characterizations of somatic hybrids developed between3 Biotech · 2017Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Costus pictus, belonging to the family Costaceae, is one of the valuable medicinal plants with its anti-diabetic property. Despite ever-increasing demand from the pharmaceutical industry, this species is being less exploited at molecular level. Hence, an effort has been made in the present study to characterize the 15 accessions of C. pictus collected from different geographical regions of India through random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. A total of 25 RAPD and 20 ISSR primers were used in the present study. The RAPD analysis generated 343 loci, of which 124 were polymorphic with an average of 4.96 loci per primer. While, ISSR primers produced 177 loci, of which 77 were polymorphic with an average of 3.85 loci per primer. The similarity coefficients ranged from 0.86-0.99, 0.84-0.95 and 0.86-0.96 for RAPD, ISSR and combined RAPD-ISSR, respectively. The UPGMA dendrogram generated using these data showed low level of divergence among the accessions from South and West regions. Further, accession-specific bands were also revealed by RAPD and ISSR markers which might be contributed to specific trait. This investigation was an understanding of genetic variation within the C. pictus accessions. The present finding indicates that both the marker tools RAPD and ISSR combined or individually can be used in determining the genetic relationship between the accessions. It may be concluded that data of hereditary differences appeared among the C. pictus accessions could be utilized for their conservation and reproducing programs.
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