Article3 Biotech2017
Effects of salt and heat pre-treatment factors on efficient regeneration in barley (Hordeum vulgare L.).
Article in 3 Biotech, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 13 citations in OpenAlex.
- Physiological responses of tomato callus and regenerated plants under PEG-induced stress during in vitro selection for drought tolerance.BMC plant biology · 2026Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An efficient callus induction and plant regeneration system has been developed using salt and heat as pre-treatment factors for three barley genotypes viz. BB-3, BB-6 and BHL-18. Different concentrations of NaCl (1.5, 2.5, 3.5, 4.5, 5.5 and 6.5 g/L) were used and its effects were determined on the basis of the viability of callus (CV), plant regeneration (PR), relative growth rate (RGR) and tolerance index (TI). The BB-6 showed highest performance on tolerance based on CV (14.72%), PR (7.69%), RGR (0.91%) and TI (0.42%) at 6.5 g/L NaCl. Various NaCl concentrations displayed significantly differences at P < 0.01 level as compared with the control. Plant regeneration capability was recorded after heat pre-treatment using calli at 30, 35 and 40 °C. In this study, BHL-18 produced highest callus induction (59.71%) after desiccated at 40 °C for BB-6. Highest regeneration was recorded around 41.66% when 4 weeks old calli were pre-treated at 35 °C. Furthermore, heat pre-treatment factors were very effective for enhancing plant regeneration (25-41.66%) which was 1.8-2.14 fold higher compared to the control (13.88-19.44%). Hence, heat treated calli displayed higher tolerance level to survive in NaCl-induced treatment for determining abiotic stress and increased regeneration rate at 35 °C temperature in BB-6 barley genotype.
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Registered trials
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