ArticleChemical science2017
Fluorogenic protein labeling using a genetically encoded unstrained alkene.
Article in Chemical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 56 citations in OpenAlex.
- In Situ Construction of Imidazopyridinium Fluorescent Labels for Bioconjugation.Angewandte Chemie (International ed. in English) · 2026Article
- A photo-enhanced oxidative coupling for site-specific protein Labeling via noncanonical amino acid incorporation.Protein engineering, design & selection : PEDS · 2026Article
- Bioorthogonal Chemistry in Biomolecule Quantification: A Review of Reactions and Strategies.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Bioorthogonal Postlabeling Reveals Nuclear Localization of a Highly Cytotoxic Half-Sandwich Ir(III) Tetrazine Complex in Live Cells.Chembiochem : a European journal of chemical biology · 2025Article
- Cracking the Code: Reprogramming the Genetic Script in Prokaryotes and Eukaryotes to Harness the Power of Noncanonical Amino Acids.Chemical reviews · 2024Review
- Structural Transformations of Metal-Organic Cages through Tetrazine-Alkene Reactivity.Journal of the American Chemical Society · 2024Article
- Proximity-enhanced protein crosslinking through an alkene-tetrazine reaction.Bioorganic chemistry · 2023Article
- Update of the Pyrrolysyl-tRNA Synthetase/tRNAJournal of bacteriology · 2023Review
- Rational Design for High Bioorthogonal Fluorogenicity of Tetrazine-Encoded Green Fluorescent Proteins.Natural sciences (Weinheim, Germany) · 2022Article
- Arylethynyltrifluoroborate Dienophiles for on Demand Activation of IEDDA Reactions.Bioconjugate chemistry · 2021Article
- A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Recent Advances in Tumor Microenvironment Hydrogen Peroxide-Responsive Materials for Cancer Photodynamic Therapy.Nano-micro letters · 2020Review
- Fitness Factors for Bioorthogonal Chemical Probes.ACS chemical biology · 2019Article
- A "Quenchergenic" Chemoselective Protein Labeling Strategy.Chembiochem : a European journal of chemical biology · 2019Article
- Bioorthogonal Fluorescence Turn-On Labeling Based on Bicyclononyne-Tetrazine Cycloaddition Reactions that Form Pyridazine Products.ChemPlusChem · 2019Article
- An Extended Approach for the Development of Fluorogenic trans-Cyclooctene-Tetrazine Cycloadditions.Chembiochem : a European journal of chemical biology · 2019Article
- A H₂O₂-Responsive Boron Dipyrromethene-Based Photosensitizer for Imaging-Guided Photodynamic Therapy.Molecules (Basel, Switzerland) · 2018Article
- Hydrophilic azaspiroalkenes as robust bioorthogonal reporters.Chemical communications (Cambridge, England) · 2018Article
- Sterically shielded tetrazoles for a fluorogenic photoclick reaction: tuning cycloaddition rate and product fluorescence.Organic & biomolecular chemistry · 2018Article
- A benzylic linker promotes methyltransferase catalyzed norbornene transfer for rapid bioorthogonal tetrazine ligation.Chemical science · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
We developed a new fluorogenic bioorthogonal reaction that is based on the inverse electron-demand Diels-Alder reaction between styrene (an unstrained alkene) and a simple tetrazine. The reaction forms a new fluorophore with no literature precedent. We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a styrene-containing amino acid into proteins in response to amber nonsense codon. Fluorogenic labeling of purified proteins and intact proteins in live cells were demonstrated. The fluorogenicity of the styrene-tetrazine reaction can be potentially applied to the study of protein folding and function under physiological conditions with low background fluorescence interference.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.