ArticleChemical science2017
Label-free target identification using in-gel fluorescence difference
Article in Chemical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Label-Free Target Discovery Strategy for Natural Active Products.Biomolecules · 2026Review
- Host-Directed Antiviral Activity of SB2960 Through Selective Induction and Remodeling of Stress Granules.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Methods for the Investigation of Protein-Ligands Interactions.Advances in experimental medicine and biology · 2026Review
- SB2301-mediated perturbation of membrane composition in lipid droplets induces lipophagy and lipid droplets ubiquitination.Communications biology · 2023Article
- Native DIGE for Quantitative and Functional Analysis of Protein Interactomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Highly effective identification of drug targets at the proteome level by pH-dependent protein precipitation.Chemical science · 2022Article
- Label-Free Target Identification Reveals the Anticancer Mechanism of a Rhenium Isonitrile Complex.Frontiers in chemistry · 2022Article
- An update of label-free protein target identification methods for natural active products.Theranostics · 2022Review
- Proteomics-based target identification of natural products affecting cancer metabolism.The Journal of antibiotics · 2021Review
- Callyspongiolide kills cells by inducing mitochondrial dysfunction via cellular iron depletion.Communications biology · 2021Article
- Phenotypic Discovery of Neuroprotective Agents by Regulation of Tau Proteostasis via Stress-Responsive Activation of PERK Signaling.Angewandte Chemie (International ed. in English) · 2021Article
- From Phenotypic Hit to Chemical Probe: Chemical Biology Approaches to Elucidate Small Molecule Action in Complex Biological Systems.Molecules (Basel, Switzerland) · 2020Review
- Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probe.Methods in enzymology · 2020Article
- Advances in exploring the therapeutic potential of marine natural products.Pharmacological research · 2019Review
- Label-free target identification reveals oxidative DNA damage as the mechanism of a selective cytotoxic agent.Chemical science · 2019Article
- Proteome-Wide Structural Biology: An Emerging Field for the Structural Analysis of Proteins on the Proteomic Scale.Journal of proteome research · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Target engagement is a prerequisite for the therapeutic effects of bioactive small molecules, and unbiased identification of their target proteins can facilitate drug discovery and chemical biology research. Structural modifications of bioactive natural products for target identification exhibit potential limitations such as synthetic difficulties, limited supplies from natural sources, and loss of original efficacy. Herein, we developed a label-free method for proteome-wide target identification using in-gel fluorescence difference caused by thermal stability shift, namely TS-FITGE. Quantitative intra-gel image analysis of each protein spot revealed target proteins with shifted thermal stability upon drug engagement, and plotting of melting curves by inter-gel analysis confirmed the positive targets. We demonstrated the robustness and applicability of the TS-FITGE method by identifying target proteins, including membrane-anchored proteins, of complex bioactive compounds. Furthermore, we identified and functionally validated nucleophosmin as a novel target protein of hordenine, a natural product upregulator of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.