ArticleChemical science2017
Ultra-specific discrimination of single-nucleotide mutations using sequestration-assisted molecular beacons.
Article in Chemical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Visualised genotyping assay with oral swabs in a closed tube by nested invasive reaction assisted with gold nanoparticle probes.IET nanobiotechnology · 2023Article
- Complexation design of cationized gelatin and molecular beacon to visualize intracellular mRNA.PloS one · 2021Article
- Enhanced SNP-sensing using DNA-templated reactions through confined hybridization of minimal substrates (CHOMS).Chemical science · 2020Article
- Target-Triggered Polymerization of Branched DNA Enables Enzyme-free and Fast Discrimination of Single-Base Changes.iScience · 2019Article
- Detection of Several Homologous MicroRNAs by a Single Smart Probe System Consisting of Linear Nucleic Acid Blockers.Molecules (Basel, Switzerland) · 2019Article
- Locus-patterned sequence oriented enrichment for multi-dimensional gene analysis.Chemical science · 2019Article
- Endonuclease IV recognizes single base mismatch on the eighth base 3' to the abasic site in DNA strands for ultra-selective and sensitive mutant-type DNA detection.RSC advances · 2018Article
- Simultaneous multiple single nucleotide polymorphism detection based on click chemistry combined with DNA-encoded probes.Chemical science · 2018Article
- Detection of low-abundance point mutations by competitive strand assisted endonuclease IV signal amplification system.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reliably distinguishing single-nucleotide mutations (SNMs) at low abundance is of great significance in clinical diagnosis. However, the specificity of most current SNM discrimination methods based on the Watson-Crick hybridization is seriously limited by the cross-reactivity of the probe with closely related unintended sequences. Herein, we propose a sequestration-assisted molecular beacon (MB) strategy for highly specific SNM discrimination. The new SNM discrimination system consists of a target-specific MB and a series of hairpin sequestering agents (SEQs). The rationally designed hairpin SEQs can effectively sequester the corresponding unintended sequences and thus dramatically improve the hybridization specificity of the MB in recognizing SNMs. The developed SNM discrimination method shows remarkably high specificity (discrimination factors ranging from 12 to 1144 with a median of 117) against 20 model SNMs, and can work rapidly and robustly over a wide range of conditions. Notably, our SNM discrimination method can be easily combined with PCR amplification for the detection of
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Registered trials
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