ArticleChemical science2017
Specific protein labeling with caged fluorophores for dual-color imaging and super-resolution microscopy in living cells.
Article in Chemical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Post-translational modification-regulating biomaterials for regeneration.Bioactive materials · 2026Review
- Article
- Synthetic fluorophores for live-cell fluorescence microscopy and biosensing.Nature chemical biology · 2025Review
- Photoactivation of BODIPY Fluorescence with Green Light.The Journal of organic chemistry · 2025Article
- β-Galactosidase- and Photo-Activatable Fluorescent Probes for Protein Labeling and Super-Resolution STED Microscopy in Living Cells.Molecules (Basel, Switzerland) · 2024Article
- Bioorthogonal Caging-Group-Free Photoactivatable Probes for Minimal-Linkage-Error Nanoscopy.ACS central science · 2023Article
- Photoactivatable BODIPYs for Live-Cell PALM.Molecules (Basel, Switzerland) · 2023Review
- 9-Cyano-10-telluriumpyronin Derivatives as Red-light-activatable Raman Probes.Chemistry, an Asian journal · 2023Article
- Implantable photonic neural probes with 3D-printed microfluidics and applications to uncaging.Frontiers in neuroscience · 2023Article
- Multifunctional stimuli-responsive chemogenetic platform for conditional multicolor cell-selective labeling.Chemical science · 2022Article
- A general design of caging-group-free photoactivatable fluorophores for live-cell nanoscopy.Nature chemistry · 2022Article
- Article
- Transcription factor dynamics in plants: Insights and technologies for in vivo imaging.Plant physiology · 2022Review
- Oxime as a general photocage for the design of visible light photo-activatable fluorophores.Chemical science · 2021Article
- Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy.Journal of the American Chemical Society · 2021Article
- Multiplexed optical barcoding of cellsChemical science · 2021Article
- In Cellulo Protein Semi-Synthesis from Endogenous and Exogenous Fragments Using the Ultra-Fast Split Gp41-1 Intein.Angewandte Chemie (International ed. in English) · 2020Article
- Dual-Activatable Cell Tracker for Controlled and Prolonged Single-Cell Labeling.ACS chemical biology · 2020Article
- tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging.Royal Society open science · 2019Article
- A guide to visualizing the spatial epigenome with super-resolution microscopy.The FEBS journal · 2019Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present new fluorophore-conjugates for dual-color photoactivation and super-resolution imaging inside live mammalian cells. These custom-designed, photo-caged Q-rhodamines and fluoresceins are cell-permeable, bright and localize specifically to intracellular targets. We utilized established orthogonal protein labeling strategies to precisely attach the photoactivatable fluorophores to proteins with subsequent activation of fluorescence by irradiation with UV light. That way, diffusive cytosolic proteins, histone proteins as well as filigree mitochondrial networks and focal adhesion proteins were visualized inside living cells. We applied the new photoactivatable probes in inverse fluorescence recovery after photo-bleaching (iFRAP) experiments, gaining real-time access to protein dynamics from live biological settings with resolution in space and time. Finally, we used the caged Q-rhodamine for photo-activated localization microscopy (PALM) on both fixed and live mammalian cells, where the superior molecular brightness and photo-stability directly resulted in improved localization precisions for different protein targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.