ArticleChemical science2017
A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva.
Article in Chemical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 98 citations in OpenAlex.
- Selection of a novel MSC-targeted aptamer Pt-1 and its functionalized hydrogel for tissue regeneration.Materials today. Bio · 2026Article
- Recent Advances in Functionally Engineered Aptamers: Strategies and Applications.Topics in current chemistry (Cham) · 2025Review
- A Novel Aptamer Biosensor Based on a Localized Surface Plasmon Resonance Sensing Chip for High-Sensitivity and Rapid Enrofloxacin Detection.Biosensors · 2023Article
- Improving aptamer performance: key factors and strategies.Mikrochimica acta · 2023Review
- A modular aldol approach for internal fluorescent molecular rotor chalcone surrogates for DNA biosensing applications.Chemical science · 2023Article
- On the Rational Design of Cooperative Receptors.Annual review of biophysics · 2023Review
- High-throughput quantitative binding analysis of DNA aptamers using exonucleases.Nucleic acids research · 2023Article
- Evaluation of probe-based ultra-sensitive detection of miRNA using a single-molecule fluorescence imaging method: miR-126 used as the model.Frontiers in bioengineering and biotechnology · 2023Article
- The sequestration mechanism as a generalizable approach to improve the sensitivity of biosensors and bioassays.Chemical science · 2022Article
- Advances and Challenges in Small-Molecule DNA Aptamer Isolation, Characterization, and Sensor Development.Angewandte Chemie (International ed. in English) · 2021Review
- Nucleic-Acid Driven Cooperative Bioassays Using Probe Proximity or Split-Probe Techniques.Analytical chemistry · 2021Review
- Thermodynamic analysis of cooperative ligand binding by the ATP-binding DNA aptamer indicates a population-shift binding mechanism.Scientific reports · 2020Article
- Rational design to control the trade-off between receptor affinity and cooperativity.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors.Nucleic acids research · 2020Review
- Engineering base-excised aptamers for highly specific recognition of adenosine.Chemical science · 2020Article
- Innovative engineering and sensing strategies for aptamer-based small-molecule detection.Trends in analytical chemistry : TRAC · 2019Article
- Sensing of cocaine using polarized optical microscopy by exploiting the conformational changes of an aptamer at the water/liquid crystal interface.Mikrochimica acta · 2019Article
- Label-Free, Visual Detection of Small Molecules Using Highly Target-Responsive Multimodule Split Aptamer Constructs.Analytical chemistry · 2019Article
- No Structure-Switching Required: A Generalizable Exonuclease-Mediated Aptamer-Based Assay for Small-Molecule Detection.Journal of the American Chemical Society · 2018Article
- FaptaSyme: A Strategy for Converting a Monomer/Oligomer-Nonselective Aptameric Sensor into an Oligomer-Selective One.Chembiochem : a European journal of chemical biology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Sensors employing split aptamers that reassemble in the presence of a target can achieve excellent specificity, but the accompanying reduction of target affinity mitigates any overall gains in sensitivity. We for the first time have developed a split aptamer that achieves enhanced target-binding affinity through cooperative binding. We have generated a split cocaine-binding aptamer that incorporates two binding domains, such that target binding at one domain greatly increases the affinity of the second domain. We experimentally demonstrate that the resulting cooperative-binding split aptamer (CBSA) exhibits higher target binding affinity and is far more responsive in terms of target-induced aptamer assembly compared to the single-domain parent split aptamer (PSA) from which it was derived. We further confirm that the target-binding affinity of our CBSA can be affected by the cooperativity of its binding domains and the intrinsic affinity of its PSA. To the best of our knowledge, CBSA-5335 has the highest cocaine affinity of any split aptamer described to date. The CBSA-based assay also demonstrates excellent performance in target detection in complex samples. Using this CBSA, we achieved specific, ultra-sensitive, one-step fluorescence detection of cocaine within fifteen minutes at concentrations as low as 50 nM in 10% saliva without signal amplification. This limit of detection meets the standards recommended by the European Union's Driving under the Influence of Drugs, Alcohol and Medicines program. Our assay also demonstrates excellent reproducibility of results, confirming that this CBSA-platform represents a robust and sensitive means for cocaine detection in actual clinical samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.