ArticleChemCatChem2017
Characterization of Carboxylic Acid Reductases as Enzymes in the Toolbox for Synthetic Chemistry.
Article in ChemCatChem, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
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- A concise enzyme cascade enables the manufacture of natural and halogenated protoberberine alkaloids.Nature communications · 2025Article
- The encoded and expressed biosynthetic potential of Greenland Ice Sheet microbes.Frontiers in microbiology · 2025Article
- Computation-driven redesign of an NRPS-like carboxylic acid reductase improves activity and selectivity.Science advances · 2024Article
- Engineering carboxylic acid reductases and unspecific peroxygenases for flavor and fragrance biosynthesis.Journal of biotechnology · 2024Review
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- Complete Genome Sequence of Nocardia iowensis DSM 45197Microbiology resource announcements · 2023Article
- Characterization of Carboxylic Acid Reductase fromPolymers · 2022Article
- A Nanoconfined Four-Enzyme Cascade Simultaneously Driven by Electrical and Chemical Energy, with Built-in Rapid, Confocal Recycling of NADP(H) and ATP.ACS catalysis · 2022Article
- α-Dioxygenases (α-DOXs): Promising Biocatalysts for the Environmentally Friendly Production of Aroma Compounds.Chembiochem : a European journal of chemical biology · 2022Review
- Biosensor and chemo-enzymatic one-pot cascade applications to detect and transform PET-derived terephthalic acid in living cells.iScience · 2022Article
- A peroxisomal heterodimeric enzyme is involved in benzaldehyde synthesis in plants.Nature communications · 2022Article
- Biocatalytic Production of Aldehydes: Exploring the Potential ofBiomolecules · 2021Article
- Fatty Acids and their Derivatives as Renewable Platform Molecules for the Chemical Industry.Angewandte Chemie (International ed. in English) · 2021Review
- Enzymkatalysierte späte Modifizierungen: Besser spät als nie.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2021Review
- Enzymatic Late-Stage Modifications: Better Late Than Never.Angewandte Chemie (International ed. in English) · 2021Review
- Microbial synthesis of vanillin from waste poly(ethylene terephthalate).Green chemistry : an international journal and green chemistry resource : GC · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carboxylic acid reductase enzymes (CARs) meet the demand in synthetic chemistry for a green and regiospecific route to aldehydes from their respective carboxylic acids. However, relatively few of these enzymes have been characterized. A sequence alignment with members of the ANL (Acyl-CoA synthetase/ NRPS adenylation domain/Luciferase) superfamily of enzymes shed light on CAR functional dynamics. Four unstudied enzymes were selected by using a phylogenetic analysis of known and hypothetical CARs, and for the first time, a thorough biochemical characterization was performed. Kinetic analysis of these enzymes with various substrates shows that they have a broad but similar substrate specificity. Electron-rich acids are favored, which suggests that the first step in the proposed reaction mechanism, attack by the carboxylate on the α-phosphate of adenosine triphosphate (ATP), is the step that determines the substrate specificity and reaction kinetics. The effects of pH and temperature provide a clear operational window for the use of these CARs, whereas an investigation of product inhibition by NADP
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