Evidence map›Paper›PMID 28450173›Full record

ArticleJournal of virological methods2017

A method for the improved detection of aerosolized influenza viruses and the male-specific (F+) RNA coliphage MS2.

J C Chandler, J W Schaeffer, M Davidson, S L Magzamen, A Pérez-Méndez, S J Reynolds, L D Goodridge, J Volckens, A B Franklin, S A Shriner and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virological methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Detection of Viruses from Bioaerosols Using Anion Exchange Resin.Journal of visualized experiments : JoVE · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 3 countries.

J C ChandlerNational Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, CO, USA.
J W SchaefferHigh Plains Intermountain Center for Agricultural Health and Safety, Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA.
M DavidsonHigh Plains Intermountain Center for Agricultural Health and Safety, Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA; Western Sydney University, Richmond, NSW, Australia.
S L MagzamenHigh Plains Intermountain Center for Agricultural Health and Safety, Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA.
A Pérez-MéndezLeprino Foods, Inc., Denver, CO, USA.
S J ReynoldsHigh Plains Intermountain Center for Agricultural Health and Safety, Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA.
L D GoodridgeDepartment of Food Science and Agricultural Chemistry, McGill University, Ste. Anne de Bellevue, QC, Canada.
J VolckensDepartment of Mechanical Engineering, Colorado State University, Fort Collins, CO, USA.
A B FranklinNational Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, CO, USA.
S A ShrinerNational Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, CO, USA.
B BishaDepartment of Animal Science, University of Wyoming, Laramie, WY, USA. Electronic address: bbisha@uwyo.edu.
Intermountain Healthcare · USAnimal and Plant Health Inspection Service · USColorado State University · USMcGill University · CAUniversity of Wyoming · US

Funding

High Plains Intermountain Center for Agricultural Health and Safety-RenewalU54OH008085 · OH · COLORADO STATE UNIVERSITY · PI STALLONES, LORANN · 2011 to 2025
$22.2M
ACL HHS U54OH008085NIOSH CDC HHS U54 OH008085
6 · The paper itself

Abstract

The detection of aerosolized viruses can serve as an important surveillance and control tool in agriculture, human health, and environmental settings. Here, we adapted an anion exchange resin-based method, initially developed to concentrate negatively charged viruses from water, to liquid impingement-based bioaerosol sampling. In this method, aerosolized viruses are collected in a 20ml liquid sample contained within widely used impingers, BioSamplers (SKC Inc., Eighty Four, PA), and further concentrated via adsorption to an anion exchange resin that is suspended within this liquid. Viral nucleic acids are then extracted from the resin to facilitate molecular analyses through a reduction in the effective sample volume. For this study, various quantities of two negatively charged viruses, type A and type B influenza viruses (FluMist Quadrivalent vaccine) and the male-specific (F+) RNA coliphage MS2 (MS2), were nebulized into a custom-built bioaerosolization chamber, and sampled using BioSamplers with and without anion exchange resin. Compared to direct testing of the BioSampler liquid, detection was improved by 6.77× and 3.33× for type A and type B influenza viruses, respectively, by using the anion exchange resin. For MS2, the anion exchange resin method allowed for an average improvement in detection of 8.26×.

Indexed as

Air MicrobiologyAerosolsAnion Exchange ResinsHumansLevivirusMaleOrthomyxoviridaeRNA, ViralSpecimen HandlingVirologyAerosolsAnion Exchange ResinsRNA, ViralAnion exchange resinBioaerosolsInfluenzaVirus concentration methods

Identifiers

PMID28450173
PMCPMC7113747
OpenAlexW2609743918

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.