Evidence map›Paper›PMID 28449669›Full record

ArticleReproductive biology and endocrinology : RB&E2017

A single trophectoderm biopsy at blastocyst stage is mathematically unable to determine embryo ploidy accurately enough for clinical use.

Norbert Gleicher, Jacob Metzger, Gist Croft, Vitaly A Kushnir, David F Albertini, David H Barad

Registry-linked trialOpen access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive biology and endocrinology : RB&E, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04744844 (Does Absence of DNA Amplification in Blastocoel Fluid Enhance Conventional Blastocyst Selection and IVF Outcome?), which is not on this map. Cited by 62 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed, 4 pooled it
20.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04744844 naterminatednot on this mapstarted 2022, after this paper: background citation

Does Absence of DNA Amplification in Blastocoel Fluid Enhance Conventional Blastocyst Selection and IVF Outcome?

TypeinterventionalSponsorAntalya IVFRan2022 to 2024Enrolled15ConditionsClinical Pregnancy, Embryo ImplantationArmsblastocoel fluid biopsy
3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 4 syntheses or guidelines pooled it, 116 citations in OpenAlex.

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2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Norbert GleicherThe Center for Human Reproduction, 21 East 69th Street, New York, NY, 10021, USA. ngleicher@thechr.com.
Jacob MetzgerCenter for Studies in Physics and Biology, The Rockefeller University, New York, NY, 10065, USA.
Gist CroftLaboratory of Stem Cell Biology and Molecular Embryology, The Rockefeller University, New York, NY, 10065, USA.
Vitaly A KushnirThe Center for Human Reproduction, 21 East 69th Street, New York, NY, 10021, USA.
David F AlbertiniThe Center for Human Reproduction, 21 East 69th Street, New York, NY, 10021, USA.
David H BaradThe Center for Human Reproduction, 21 East 69th Street, New York, NY, 10021, USA.
Center for Human Reproduction · USRockefeller University · USWake Forest University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIt has become increasingly apparent that the trophectoderm (TE) at blastocyst stage is much more mosaic than has been appreciated. Whether preimplantation genetic screening (PGS), utilizing a single TE biopsy (TEB), can reliably determine embryo ploidy has, therefore, increasingly been questioned in parallel.

methodsWe for that reason here established 2 mathematical models to assess probabilities of false-negative and false-positive results of an on average 6-cell biopsy from an approximately 300-cell TE. This study was a collaborative effort between investigators at The Center for Human Reproduction in New York City and the Center for Studies in Physics and Biology and the Brivanlou Laboratory of Stem Cell Biology and Molecular Embryology, the latter two both at Rockefeller University in New York City.

resultsBoth models revealed that even under best case scenario, assuming even distribution of mosaicism in TE (since mosaicism is usually clonal, a highly unlikely scenario), a biopsy of at least 27 TE cells would be required to reach minimal diagnostic predictability from a single TEB.

conclusionsAs currently performed, a single TEB is, therefore, mathematically incapable of reliably determining whether an embryo can be transferred or should be discarded. Since a single TEB, as currently performed, apparently is not representative of the complete TE, this study, thus, raises additional concern about the clinical utilization of PGS.

Indexed as

BlastocystCleavage Stage, OvumPloidiesAneuploidyBiopsyEctodermEmbryo ImplantationFemaleHumansModels, TheoreticalPregnancyPreimplantation DiagnosisReproducibility of ResultsTrophoblastsIn vitro fertilizationMathematical modelPremplantation genetic screeningTrophectoderm biopsy

Identifiers

PMID28449669
PMCPMC5408377
OpenAlexW2609225490

What OpenQuestion holds

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LicenceCC BY
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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.