ArticleNucleic acids research2017
Using high-throughput barcode sequencing to efficiently map connectomes.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits.Nature communications · 2026Article
- Synaptic activity controls local exposure of an 'eat-me' signal via ANO3-ITPR1 signaling.bioRxiv : the preprint server for biology · 2026Article
- Synaptic connectomics by sequencing.Nature methods · 2026Article
- Connectome-seq: high-throughput mapping of neuronal connectivity at single-synapse resolution via barcode sequencing.Nature methods · 2026Article
- Synaptic-resolution connectomics: towards large brains and connectomic screening.Nature reviews. Neuroscience · 2026Review
- Connectome-seq: High-throughput Mapping of Neuronal Connectivity at Single-Synapse Resolution via Barcode Sequencing.bioRxiv : the preprint server for biology · 2025Article
- The diversification of methods for studying cell-cell interactions and communication.Nature reviews. Genetics · 2024Review
- Integrating barcoded neuroanatomy with spatial transcriptional profiling enables identification of gene correlates of projections.Nature neuroscience · 2021Article
- Sequencing-Based High-Throughput Neuroanatomy: From Mapseq to Bricseq and Beyond.Neuroscience bulletin · 2021Article
- Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems.Science (New York, N.Y.) · 2021Article
- Human stem cell-based models for studying autism spectrum disorder-related neuronal dysfunction.Molecular autism · 2020Review
- SYNPLA, a method to identify synapses displaying plasticity after learning.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Bringing Microscopy-By-Sequencing into View.Trends in biotechnology · 2020Review
- A ligand-based system for receptor-specific delivery of proteins.Scientific reports · 2019Article
- A computational framework for DNA sequencing microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Reprogramming the brain with synthetic neurobiology.Current opinion in biotechnology · 2019Review
- Perspective of Molecular Diagnosis in Healthcare: From Barcode to Pattern Recognition.Diagnostics (Basel, Switzerland) · 2019Article
- Network cloning using DNA barcodes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Measuring sequencer size bias using REcount: a novel method for highly accurate Illumina sequencing-based quantification.Genome biology · 2019Article
- Cellular barcoding: lineage tracing, screening and beyond.Nature methods · 2018Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The function of a neural circuit is determined by the details of its synaptic connections. At present, the only available method for determining a neural wiring diagram with single synapse precision-a 'connectome'-is based on imaging methods that are slow, labor-intensive and expensive. Here, we present SYNseq, a method for converting the connectome into a form that can exploit the speed and low cost of modern high-throughput DNA sequencing. In SYNseq, each neuron is labeled with a unique random nucleotide sequence-an RNA 'barcode'-which is targeted to the synapse using engineered proteins. Barcodes in pre- and postsynaptic neurons are then associated through protein-protein crosslinking across the synapse, extracted from the tissue, and joined into a form suitable for sequencing. Although our failure to develop an efficient barcode joining scheme precludes the widespread application of this approach, we expect that with further development SYNseq will enable tracing of complex circuits at high speed and low cost.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.