Evidence map›Paper›PMID 28448718›Full record

ArticleThe American journal of drug and alcohol abuse2017

Cannabis use by women during pregnancy does not influence infant DNA methylation of the dopamine receptor DRD4.

Peter D Fransquet, Delyse Hutchinson, Craig A Olsson, Steve Allsop, Elizabeth J Elliott, Lucinda Burns, Richard Mattick, Richard Saffery, Joanne Ryan

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of drug and alcohol abuse, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 15 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Review
  5. Consequences of Perinatal Cannabis Exposure.Trends in neurosciences · 2019
    Review
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 2 countries.

Peter D Fransqueta Murdoch Childrens Research Institute, The University of Melbourne , Parkville , Victoria , Australia.
Delyse Hutchinsona Murdoch Childrens Research Institute, The University of Melbourne , Parkville , Victoria , Australia.
Craig A Olssona Murdoch Childrens Research Institute, The University of Melbourne , Parkville , Victoria , Australia.
Steve Allsope National Drug Research Institute , Curtin University , Perth , Australia.
Elizabeth J Elliottf Discipline of Paediatrics and Child Health , The University of Sydney, The Sydney Children's Hospital's Network (Westmead) , Sydney , Australia.
Lucinda Burnsb Department of Paediatrics , University of Melbourne, Royal Children's Hospital , Melbourne , Australia.
Richard Mattickd National Drug and Alcohol Research Centre, University of New South Wales , Sydney , Australia.
Richard Safferya Murdoch Childrens Research Institute, The University of Melbourne , Parkville , Victoria , Australia.
Joanne Ryana Murdoch Childrens Research Institute, The University of Melbourne , Parkville , Victoria , Australia.
University of Melbourne · AURoyal Children's Hospital · AUCurtin University · AUInserm · FRSydney Children’s Hospitals Network · AUUNSW Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMaternal cannabis use in pregnancy is linked with long-term adverse behavioral outcomes in offspring. Epigenetic processes established in utero that affect dopaminergic (reward) signaling may mediate risks. Associations between cannabis use and offspring DNA methylation have not been investigated; however, maternal tobacco smoking in pregnancy is associated with distinct patterns of DNA methylation at birth and beyond.

objectivesTo determine whether maternal cannabis use is associated with methylation of the dopamine receptor gene DRD4 promoter in infants.

methodsMothers in the Triple B study provided detailed information on drug use in each trimester of pregnancy. Buccal swabs were collected from neonates at 8 weeks (n = 804, 51.7% male, and 48.3% female). DRD4 promoter DNA methylation was measured using SEQUENOM MassARRAY.

resultsFifty-seven of the women in the study reported drug use during pregnancy, of whom 44 used cannabis. Of 19 cytosine-phosphate-guanine dinucleotides (CpG) units tested in DRD4, gestational cannabis use was associated with offspring methylation at 1 CpG unit in multivariate models (β + 1.48, CI: 0.02 to 2.93, and p = 0.047). At another site there was weak evidence that both cannabis and other drug use were independently associated with increased methylation, while the association with tobacco was in the reverse direction (cannabis use β + 0.67, CI: -0.12 to 1.46, and p = 0.09; other drug use β + 1.11, CI: 0.17 to 2.05, and p = 0.02; tobacco use β -0.41, CI: -0.85 to 0.03, and p = 0.07). None of the associations would remain significant after correction for multiple testing.

conclusionThere is no strong evidence that maternal cannabis use in pregnancy is associated with offspring DRD4 methylation.

Indexed as

AdultDNA MethylationFemaleHumansInfantMaleMarijuana SmokingPregnancyPrenatal Exposure Delayed EffectsPromoter Regions, GeneticReceptors, DopamineTobacco SmokingYoung AdultReceptors, DopamineCannabisDNA methylationdopamine receptor (DRD4)epigeneticsfetal programmingperinatalpregnancy

Identifiers

PMID28448718
PMCPMC5706968
OpenAlexW2608305951

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.