ArticleJournal of visualized experiments : JoVE2017
Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Phenotypic and functional characterization of first-trimester human placental macrophages, Hofbauer cells.The Journal of experimental medicine · 2021Trial
- A subset of neutrophil phagosomes is characterised by pulses of Class I PI3K activity.Disease models & mechanisms · 2025Article
- Exposure ofThe Journal of biological chemistry · 2019Article
- Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte Subsets.Frontiers in immunology · 2019Article
- Heterogeneity of hypochlorous acid production in individual neutrophil phagosomes revealed by a rhodamine-based probe.The Journal of biological chemistry · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Neutrophils are crucial to host innate defense and, consequently, constitute an important area of medical research. The phagosome, the intracellular compartment where the killing and digestion of engulfed particles take place, is the main arena for neutrophil pathogen killing that requires tight regulation. Phagosomal pH is one aspect that is carefully controlled, in turn regulating antimicrobial protease activity. Many fluorescent pH-sensitive dyes have been used to visualize the phagosomal environment. S-1 has several advantages over other pH-sensitive dyes, including its dual emission spectra, its resistance to photo-bleaching, and its high pKa. Using this method, we have demonstrated that the neutrophil phagosome is unusually alkaline in comparison to other phagocytes. By using different biochemical conjugations of the dye, the phagosome can be delineated from the cytoplasm so that changes in the size and shape of the phagosome can be assessed. This allows for further monitoring of ionic movement.
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Registered trials
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