Evidence map›Paper›PMID 28448016›Full record

ArticleJournal of visualized experiments : JoVE2017

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons.

Rosa L Moreno, Megan Josey, Angeles B Ribera

Open access · bronzeAbstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Rosa L MorenoDepartment of Physiology and Biophysics, University of Colorado Anschutz Medical Campus (UCAMC); Rosa.Moreno@ucdenver.edu.
Megan JoseyNeuroscience Graduate Program, University of Colorado Anschutz Medical Campus (UCAMC).
Angeles B RiberaDepartment of Physiology and Biophysics, University of Colorado Anschutz Medical Campus (UCAMC); Neuroscience Graduate Program, University of Colorado Anschutz Medical Campus (UCAMC).
University of Colorado Anschutz Medical Campus · US

Funding

ZEBRAFISH TRANSGENIC CORE - ROCKY MOUNTAIN NEUROLOGICAL DISORDERS CORE CENTERP30NS048154 · NINDS · UNIVERSITY OF COLORADO DENVER · PI RIBERA, ANGELES BADELL · 2004 to 2020
$10.1M
MOLECULAR ANALYSIS OF K+CURRENTS IN DEVELOPING NEURONSR01NS025217 · NINDS · UNIVERSITY OF COLORADO DENVER · PI RIBERA, ANGELES BADELL · 1993 to 2014
$3.9M
Neuronal Transdifferention in vivo: Mechanism and PotentialR01NS086839 · NINDS · UNIVERSITY OF COLORADO DENVER · PI RIBERA, ANGELES BADELL · 2014 to 2018
$1.7M
Motor neuron subtype-specific development of excitability in vivoF32NS059120 · NINDS · UNIVERSITY OF COLORADO DENVER · PI MORENO, ROSA L · 2007 to 2009
$172k
NINDS NIH HHS F32 NS059120NINDS NIH HHS P30 NS048154NINDS NIH HHS R01 NS025217NINDS NIH HHS R01 NS086839
6 · The paper itself

Abstract

Zebrafish, first introduced as a developmental model, have gained popularity in many other fields. The ease of rearing large numbers of rapidly developing organisms, combined with the embryonic optical clarity, served as initial compelling attributes of this model. Over the past two decades, the success of this model has been further propelled by its amenability to large-scale mutagenesis screens and by the ease of transgenesis. More recently, gene-editing approaches have extended the power of the model. For neurodevelopmental studies, the zebrafish embryo and larva provide a model to which multiple methods can be applied. Here, we focus on methods that allow the study of an essential property of neurons, electrical excitability. Our preparation for the electrophysiological study of zebrafish spinal neurons involves the use of veterinarian suture glue to secure the preparation to a recording chamber. Alternative methods for recording from zebrafish embryos and larvae involve the attachment of the preparation to the chamber using a fine tungsten pin

Indexed as

ZebrafishAnimalsDissectionEmbryo, NonmammalianInterneuronsLarvaMotor NeuronsNeuronsPatch-Clamp TechniquesSensory Receptor CellsSpinal Cord

Identifiers

PMID28448016
PMCPMC5564991
OpenAlexW2606354971

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.