Evidence map›Paper›PMID 28447752›Full record

ArticleMolecular medicine reports2017

Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1.

Qin Yi, Hao Xu, Ke Yang, Yue Wang, Bin Tan, Jie Tian, Jing Zhu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 21 citations in OpenAlex.

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  10. Myocardial tissue-specific Dnmt1 knockout in rats protects against pathological injury induced by Adriamycin.Laboratory investigation; a journal of technical methods and pathology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Qin YiDepartment of Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Hao XuDepartment of Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Ke YangDepartment of Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Yue WangDepartment of Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Bin TanDepartment of Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Jie TianCardiovascular Department (Internal Medicine), Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Jing ZhuDepartment of Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Chongqing Medical University · CNChildren's Hospital of Chongqing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Previous studies from this group demonstrated that insulin gene enhancer binding protein ISL-1 (Islet-1) specifically induces the differentiation of mesenchymal stem cells (MSCs) into cardiomyocyte‑like cells through histone acetylation. However, the underlying mechanisms remain unclear. In the present study, the role of the histone acetylation and DNA methylation on the regulatory mechanism of the Islet‑1 was further investigated by methylation‑specific polymerase chain reaction (PCR), chromatin immunoprecipitation quantitative PCR and western blot analysis. The results demonstrated that Islet‑1 upregulated expression of general control of amino acid biosynthesis protein 5 (Gcn5) and enhanced the binding of Gcn5 to the promoters of GATA binding protein 4 (GATA4) and NK2 homeobox 5 (Nkx2.5). In addition, Islet-1 downregulated DNA methyltransferase (DNMT)‑1 expression and reduced its binding to the GATA4 promoter. In contrast, the amount of DNMT-1 binding on Nkx2.5 did not match the expression trend. Therefore, it was concluded that Islet‑1 may influence the histone acetylation and DNA methylation of GATA4 promoter region via Gcn5 and DNMT‑1 during the MSC differentiation into cardiomyocyte-like cells, thus prompting the expression of GATA4. The Nkx2.5 was likely only affected by histone acetylation instead of DNA methylation. The present study demonstrated that Islet‑1 induces the differentiation of mesenchymal stem cells into cardiomyocyte‑like cells through a specific interaction between histone acetylation and DNA methylation on regulating GATA4.

Indexed as

AnimalsCell DifferentiationCell LineDNA (Cytosine-5-)-Methyltransferase 1LIM-Homeodomain ProteinsMesenchymal Stem CellsMiceMyocytes, Cardiacp300-CBP-Associated Factorp300-CBP Transcription FactorsTranscription FactorsDNA (Cytosine-5-)-Methyltransferase 1Dnmt1 protein, mouseinsulin gene enhancer binding protein Isl-1LIM-Homeodomain Proteinsp300-CBP-Associated Factorp300-CBP Transcription FactorsTranscription Factors

Identifiers

PMID28447752
PMCPMC5428324
OpenAlexW2595137847

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.