ArticleMicrobial biotechnology2017
Transcriptional response of Escherichia coli to ammonia and glucose fluctuations.
Article in Microbial biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Hyperammonemia reduces the populations of beneficial lactobacilli and bifidobacteria, disrupting the metabolic balance of the gut microbiome in rats.Frontiers in microbiology · 2026Article
- A mismatch between slow protein synthesis and fast environmental fluctuations determines tradeoffs in bacterial proteome allocation strategies.bioRxiv : the preprint server for biology · 2025Article
- Impact of mixing insufficiencies on L-phenylalanine production with an Escherichia coli reporter strain in a novel two-compartment bioreactor.Microbial cell factories · 2023Article
- Relationship between Nonhepatic Serum Ammonia Levels and Sepsis-Associated Encephalopathy: A Retrospective Cohort Study.Emergency medicine international · 2023Article
- Application of anEngineering in life sciences · 2023Article
- Impact of Elevated Levels of Dissolved COMicroorganisms · 2022Article
- Live-Cell Imaging of Guanosine Tetra- and Pentaphosphate (p)ppGpp with RNA-based Fluorescent Sensors*.Angewandte Chemie (International ed. in English) · 2021Article
- Transcriptional profiling of the stringent response mutant strain E. coli SR reveals enhanced robustness to large-scale conditions.Microbial biotechnology · 2021Article
- Euler-Lagrangian Simulations: A Proper Tool for Predicting Cellular Performance in Industrial Scale Bioreactors.Advances in biochemical engineering/biotechnology · 2021Article
- Potential of Integrating Model-Based Design of Experiments Approaches and Process Analytical Technologies for Bioprocess Scale-Down.Advances in biochemical engineering/biotechnology · 2021Article
- Pseudomonas putida KT2440 is naturally endowed to withstand industrial-scale stress conditions.Microbial biotechnology · 2020Article
- Development and characterization of Escherichia coli triple reporter strains for investigation of population heterogeneity in bioprocesses.Microbial cell factories · 2020Article
- Comparative performance of different scale-down simulators of substrate gradients in Penicillium chrysogenum cultures: the need of a biological systems response analysis.Microbial biotechnology · 2018Article
- The effect of metabolic stress on genome stability of a synthetic biology chassis Escherichia coli K12 strain.Microbial cell factories · 2018Article
- In Silico Prediction of Large-Scale Microbial Production Performance: Constraints for Getting Proper Data-Driven Models.Computational and structural biotechnology journal · 2018Review
- Bioprocess scale-up/down as integrative enabling technology: from fluid mechanics to systems biology and beyond.Microbial biotechnology · 2017Article
- Scale-up/Scale-down of microbial bioprocesses: a modern light on an old issue.Microbial biotechnology · 2017Article
- Transcriptional response of Escherichia coli to ammonia and glucose fluctuations.Microbial biotechnology · 2017Article
- Repetitive Short-Term Stimuli Imposed in Poor Mixing Zones Induce Long-Term Adaptation ofFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In large-scale production processes, metabolic control is typically achieved by limited supply of essential nutrients such as glucose or ammonia. With increasing bioreactor dimensions, microbial producers such as Escherichia coli are exposed to changing substrate availabilities due to limited mixing. In turn, cells sense and respond to these dynamic conditions leading to frequent activation of their regulatory programmes. Previously, we characterized short- and long-term strategies of cells to adapt to glucose fluctuations. Here, we focused on fluctuating ammonia supply while studying a continuously running two-compartment bioreactor system comprising a stirred tank reactor (STR) and a plug-flow reactor (PFR). The alarmone ppGpp rapidly accumulated in PFR, initiating considerable transcriptional responses after 70 s. About 400 genes were repeatedly switched on/off when E. coli returned to the STR. E. coli revealed highly diverging long-term transcriptional responses in ammonia compared to glucose fluctuations. In contrast, the induction of stringent regulation was a common feature of both short-term responses. Cellular ATP demands for coping with fluctuating ammonia supply were found to increase maintenance by 15%. The identification of genes contributing to the increased ATP demand together with the elucidation of regulatory mechanisms may help to create robust cells and processes for large-scale application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.