Evidence map›Paper›PMID 28446066›Full record

ArticleStem cells and development2017

A Novel Three-Dimensional Culture System for Oligodendrocyte Precursor Cells.

Naohiro Egawa, Akihiro Shindo, Anna C Liang, Yang Du, Changhong Xing, Evan K Lo, Kanako Itoh, Hisanori Kinoshita, Takakuni Maki, Ryosuke Takahashi and 4 more

Abstract read
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In one paragraph

Article in Stem cells and development, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.4field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 3 countries.

Naohiro Egawa1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts.
Akihiro Shindo1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts.
Anna C Liang1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts.
Yang Du1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts.
Changhong Xing1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts.
Evan K Lo1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts.
Kanako Itoh1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts.
Hisanori Kinoshita3 Department of Neurology, Graduate School of Medicine, Kyoto University , Kyoto, Japan .
Takakuni Maki3 Department of Neurology, Graduate School of Medicine, Kyoto University , Kyoto, Japan .
Ryosuke Takahashi3 Department of Neurology, Graduate School of Medicine, Kyoto University , Kyoto, Japan .
Ryo Sudo4 Department of System Design Engineering, Keio University , Yokohama, Japan .
Myron Spector5 Tissue Engineering, VA Boston Healthcare System, Brigham and Women's Hospital , Harvard Medical School, Boston, Massachusetts.
Josephine Lok1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts.
Ken Arai1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts.
Harvard University · USMassachusetts General Hospital · USKyoto University · JPKeio University · JPXiangya Hospital Central South University · CN

Funding

Targeting ROCK for Neurogliovascular Plasticity & Remodeling After StrokeP01NS055104 · NINDS · MASSACHUSETTS GENERAL HOSPITAL · PI LO, ENG H. · 2007 to 2018
$13.3M
NINDS NIH HHS P01 NS055104
6 · The paper itself

Abstract

Oligodendrocytes are generated from oligodendrocyte precursor cells (OPCs). Mechanisms of OPC differentiation have been extensively examined with two-dimensional cell culture systems. However, these cellular events may be more accurately represented using a three-dimensional (3D) model. In this study, we report the development of a novel 3D OPC culture system using gels composed of a mixture of collagen and hyaluronan, wherein cultured rat primary OPCs can proliferate and differentiate into oligodendrocytes. Our data show that the gel concentration and cell-seeding density are critical factors for the numbers of OPCs and oligodendrocytes in our 3D culture system. In addition, Notch signaling, which supports cell-to-cell communication, may also be important for OPC function in our system because a Notch inhibitor DAPT suppressed OPC proliferation and differentiation. Taken together, cultured rat OPCs can grow in collagen-/hyaluronan-based gels, and our novel 3D OPC culture system may offer a useful platform for examining the mechanisms of OPC function in vitro.

Indexed as

AnimalsAstrocytesCell CountCell Culture TechniquesCell DifferentiationCell LineageCell ProliferationCells, CulturedGelsOligodendrocyte Precursor CellsPorosityRats, Sprague-DawleyReceptors, NotchSignal TransductionGelsReceptors, Notch3D cultureastrocytecell–cell interactionNotch signalingoligodendrocyte precursor cell

Identifiers

PMID28446066
PMCPMC5512301
OpenAlexW2609196261

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.