ArticleBiophysical journal2017
Membrane Mechanics of Primary Afferent Neurons in the Dorsal Root Ganglia of Rats.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Label-Free Visualization and Morphological Profiling of Neuronal Differentiation and Axonal Degeneration through Quantitative Phase Imaging.Advanced biology · 2024Article
- Engineering a multicompartment in vitro model for dorsal root ganglia phenotypic assessment.Journal of biomedical materials research. Part B, Applied biomaterials · 2023Article
- IGF-1 Combined with OPN Promotes Neuronal Axon Growth in Vitro Through the IGF-1R/Akt/mTOR Signaling Pathway in Lipid Rafts.Neurochemical research · 2023Article
- Potentiation of PIEZO2 mechanically-activated currents in sensory neurons mediates vincristine-induced mechanical hypersensitivity.Acta pharmaceutica Sinica. B · 2023Article
- Rapid propagation of membrane tension at retinal bipolar neuron presynaptic terminals.Science advances · 2022Article
- Molecular dynamics simulations of Piezo1 channel opening by increases in membrane tension.Biophysical journal · 2021Article
- Substrate Stiffness Mediates Formation of Novel Cytoskeletal Structures in Fibroblasts during Cell-Microspheres Interaction.International journal of molecular sciences · 2021Article
- Viscoelasticity and Volume of Cortical Neurons under Glutamate Excitotoxicity and Osmotic Challenges.Biophysical journal · 2020Article
- Pay attention to membrane tension: Mechanobiology of the cell surface.Current opinion in cell biology · 2020Review
- T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration.Nature communications · 2020Article
- Axonal Transport, Phase-Separated Compartments, and Neuron Mechanics - A New Approach to Investigate Neurodegenerative Diseases.Frontiers in cellular neuroscience · 2018Review
- Deterministic Integration of Biological and Soft Materials onto 3D Microscale Cellular Frameworks.Advanced biosystems · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Membrane mechanics is an important biological factor regulating many cellular functions including cell motility, intercellular and intracellular signaling, gene expression, and membrane ion channel activity. Primary afferent neurons transduce sensory information about temperature, touch, and pain. These sensory functions may be profoundly affected by the states of primary afferent neuron mechanics. However, membrane mechanics of primary afferent neurons is largely unknown. In this study, we established the optical trapping technique for determining membrane mechanics of cultured primary afferent neurons of the dorsal root ganglia (DRG). We further determined the roles of cytoskeleton and membrane lipids in DRG neuron mechanics. We found that DRG neurons had a plasma membrane tension of ∼54 pN/μm, and the tension was significantly decreased to ∼29 pN/μm by cytochalasin D treatment to disrupt actin cytoskeleton and increased to ∼79 pN/μm by methyl-β-cyclodextrin treatment to sequester membrane cholesterol. DRG neuron membrane stiffness was not significantly affected by the cytoskeleton disruption but was significantly increased after cholesterol sequestration. Our findings elucidate membrane mechanical properties of primary afferent neurons, which provide, to our knowledge, a new perspective on their sensory functions.
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