ArticleCytoskeleton (Hoboken, N.J.)2017
High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications.
Article in Cytoskeleton (Hoboken, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 60 citations in OpenAlex.
- Simulation-Based Microfluidic Deformation Mapping for Region-Dependent Apparent Young's Modulus Estimation of Single Cells.Biosensors · 2026Article
- High-throughput mechanomic screening reveals novel regulators of single-cell mechanics.Biophysical journal · 2026Article
- B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses.FEBS letters · 2025Review
- Viscoelastic recovery times of chondrocytes measured using a novel 3D-printed microfluidic device.Measurement science & technology · 2025Article
- Real-time viscoelastic deformability cytometry: High-throughput mechanical phenotyping of liquid and solid biopsies.Science advances · 2024Article
- Single-cell electro-mechanical shear flow deformability cytometry.Microsystems & nanoengineering · 2024Article
- Article
- High-throughput mechano-cytometry as a method to detect apoptosis, necroptosis, and ferroptosis.Cell proliferation · 2023Article
- Multimodal microscale mechanical mapping of cancer cells in complex microenvironments.Biophysical journal · 2022Article
- Interpretation of cell mechanical experiments in microfluidic systems depend on the choice of cellular shape descriptors.Biomicrofluidics · 2022Article
- Machine learning assisted real-time deformability cytometry of CD34+ cells allows to identify patients with myelodysplastic syndromes.Scientific reports · 2022Article
- Deep learning assisted mechanotyping of individual cells through repeated deformations and relaxations in undulating channels.Biomicrofluidics · 2022Article
- A comparison of microfluidic methods for high-throughput cell deformability measurements.Nature methods · 2020Article
- High-throughput cell and spheroid mechanics in virtual fluidic channels.Nature communications · 2020Article
- Physical Biomarkers of Disease Progression: On-Chip Monitoring of Changes in Mechanobiology of Colorectal Cancer Cells.Scientific reports · 2020Article
- Effect of Nuclear Stiffness on Cell Mechanics and Migration of Human Breast Cancer Cells.Frontiers in cell and developmental biology · 2020Article
- Maturation of Monocyte-Derived DCs Leads to Increased Cellular Stiffness, Higher Membrane Fluidity, and Changed Lipid Composition.Frontiers in immunology · 2020Article
- Microfluidic deformability-activated sorting of single particles.Microsystems & nanoengineering · 2020Article
- Cardiomyocyte mechanodynamics under conditions of actin remodelling.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Cells Under Stress: An Inertial-Shear Microfluidic Determination of Cell Behavior.Biophysical journal · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanical fingerprint of cells is inherently linked to the structure of the cytoskeleton and can serve as a label-free marker for cell homeostasis or pathologic states. How cytoskeletal composition affects the physical response of cells to external loads has been intensively studied with a spectrum of techniques, yet quantitative and statistically powerful investigations in the form of titration assays are hampered by the low throughput of most available methods. In this study, we employ real-time deformability cytometry (RT-DC), a novel microfluidic tool to examine the effects of biochemically modified F-actin and microtubule stability and nuclear chromatin structure on cell deformation in a human leukemia cell line (HL60). The high throughput of our method facilitates extensive titration assays that allow for significance assessment of the observed effects and extraction of half-maximal concentrations for most of the applied reagents. We quantitatively show that integrity of the F-actin cortex and microtubule network dominate cell deformation on millisecond timescales probed with RT-DC. Drug-induced alterations in the nuclear chromatin structure were not found to consistently affect cell deformation. The sensitivity of the high-throughput cell mechanical measurements to the cytoskeletal modifications we present in this study opens up new possibilities for label-free dose-response assays of cytoskeletal modifications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.