ArticlePloS one2017
Shoot stem cell specification in roots by the WUSCHEL transcription factor.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- A Comprehensive Analysis on the Regulatory Network Underlying Callus Induction and Adventitious Organogenesis Process in Stem ofInternational journal of molecular sciences · 2025Article
- De novo root regeneration from leaf explant: a mechanistic review of key factors behind cell fate transition.Planta · 2025Review
- Success and limitations in adaptation of Fast-TrACC tissue culture-independent transformation in coffee, cotton, and tree tobacco.PloS one · 2025Article
- ABA inhibits in vitro shoot regeneration by affecting H3K9ac modification of WUS in Arabidopsis.Protoplasma · 2024Article
- Plant developmental oddities.Planta · 2024Review
- Plant regeneration in the new era: from molecular mechanisms to biotechnology applications.Science China. Life sciences · 2024Review
- CDC48A, an interactor of WOX2, is required for embryonic patterning in Arabidopsis thaliana.Plant cell reports · 2024Article
- How does light regulate plant regeneration?Frontiers in plant science · 2024Review
- Epigenetic modifications and miRNAs determine the transition of somatic cells into somatic embryos.Plant cell reports · 2023Review
- Review
- Maintenance of stem cell activity in plant development and stress responses.Frontiers in plant science · 2023Review
- Overexpression of thePlant signaling & behavior · 2022Article
- Genetic and epigenetic modes of the regulation of somatic embryogenesis: a review.Biologia futura · 2022Review
- Wuschel2 enables highly efficient CRISPR/Cas-targeted genome editing during rapid de novo shoot regeneration in sorghum.Communications biology · 2022Article
- Molecular Determinants ofFrontiers in plant science · 2022Review
- Review
- Natural Variation in Plant Pluripotency and Regeneration.Plants (Basel, Switzerland) · 2020Review
- WUSCHEL: a master regulator in plant growth signaling.Plant cell reports · 2020Review
- WUSCHEL acts as an auxin response rheostat to maintain apical stem cells in Arabidopsis.Nature communications · 2019Article
- Signaling Overview of Plant Somatic Embryogenesis.Frontiers in plant science · 2019Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The WUSCHEL homeobox transcription factor is required to specify stem-cell identity at the shoot apical meristem and its ectopic expression is sufficient to induce de novo shoot meristem formation. Yet, the manner by which WUS promotes stem-cell fate is not yet fully understood. In the present research we address this question by inducing WUS function outside of its domain. We show that activation of WUS function in the root inhibits the responses to exogenous auxin and suppresses the initiation and growth of lateral roots. Using time lapse movies to follow the cell-cycle marker CYCB1;1::GFP, we also show that activation of WUS function suppresses cell division and cell elongation. In addition, activation of WUS represses the auxin-induced expression of the PLETHORA1 root identity gene and promotes shoot fate. Shoot apical meristem formation requires a high cytokinin-to-auxin ratio. Our findings provide evidence for the manner by which WUS specifies stem-cell identity: by affecting auxin responses, by reducing the cell mitotic activity and by repressing other developmental pathways. At the meristem, the stem-cells which are characterized by low division rate are surrounded by the highly proliferative meristematic cells. Our results also provide a model for WUS establishing the differential mitotic rates between two cell populations at the minute structure of the meristem.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.