ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2017
Large population cell characterization using quantitative phase cytometer.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- High-speed laser-scanning biological microscopy using FACED.Nature protocols · 2021Article
- Label-free imaging flow cytometer for analyzing large cell populations by line-field quantitative phase microscopy with digital refocusing.Biomedical optics express · 2020Article
- Multi-ATOM: Ultrahigh-throughput single-cell quantitative phase imaging with subcellular resolution.Journal of biophotonics · 2019Article
- Real-time halo correction in phase contrast imaging.Biomedical optics express · 2018Article
- Dynamic spatial filtering using a digital micromirror device for high-speed optical diffraction tomography.Optics express · 2018Article
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Authors and funding
6 authors.
Funding
Abstract
A major challenge in cellular analysis is the phenotypic characterization of large cell populations within a short period of time. Among various parameters for cell characterization, the cell dry mass is often used to describe cell size but is difficult to be measured directly with traditional techniques. Here, we propose an interferometric approach based on line-focused beam illumination for high-content precision dry mass measurements of adherent cells in a non-invasive fashion-we call it quantitative phase cytometry (QPC). Besides dry mass, abundant cellular morphological features such as projected area, sphericity, and phase skewness can be readily extracted from the QPC interferometric data. To validate the utility of our technique, we demonstrate characterizing a large population of ∼10
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