ArticleThe Journal of veterinary medical science2017
Deep sequencing of the transcriptome in the anterior pituitary of heifers before and after ovulation.
Article in The Journal of veterinary medical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- A novel method of correlative light and electron microscopy in cryosectioning of bovine anterior pituitary tissue using NanoSuit CLEM.The Journal of reproduction and development · 2025Article
- Association of Metabolic and Endocrine Disorders with Bovine Ovarian Follicular Cysts.Animals : an open access journal from MDPI · 2023Article
- Differences in Milk Proteomic Profiles between Estrous and Non-Estrous Dairy Cows.Animals : an open access journal from MDPI · 2023Article
- Age-associated changes in gene expression in the anterior pituitary glands of female Japanese black cattle.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Article
- Reduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains.Scientific reports · 2021Article
- Discovery of new receptors regulating luteinizing hormone and follicle-stimulating hormone secretion by bovine gonadotrophs to explore a new paradigm for mechanisms regulating reproduction.The Journal of reproduction and development · 2020Review
- Article
- Genome Analysis Reveals Genetic Admixture and Signature of Selection for Productivity and Environmental Traits in Iraqi Cattle.Frontiers in genetics · 2019Article
- A novel somatic mutation of SIN3A detected in breast cancer by whole-exome sequencing enhances cell proliferation through ERα expression.Scientific reports · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We aimed to determine gene expression patterns in the anterior pituitary (AP) of heifers before and after ovulation via deep sequencing of the transcriptome (RNA-seq) to identify new genes and clarify important pathways. Heifers were slaughtered on the estrus day (pre-ovulation; n=5) or 3 days after ovulation (post-ovulation; n=5) for AP collection. We randomly selected 4 pre-ovulation and 4 post-ovulation APs, and the ribosomal RNA-depleted poly (A)+RNA were prepared to assemble next-generation sequencing libraries. The bovine APs expressed 12,769 annotated genes at pre- or post-ovulation. The sum of the reads per kilobase of exon model per million mapped reads (RPKM) values of all transcriptomes were 599,676 ± 38,913 and 668,209 ± 23,690, and 32.2 ± 2.6% and 44.0 ± 4.4% of these corresponded to the AP hormones in the APs of pre- and post-ovulation heifers, respectively. The bovine AP showed differential expression of 396 genes (P<0.05) in the pre- and post-ovulation APs. The 396 genes included two G-protein-coupled receptor (GPCR) genes (GPR61 and GPR153) and those encoding 13 binding proteins. The AP also expressed 259 receptor and other 364 binding proteins. Moreover, ingenuity pathway analysis for the 396 genes revealed (P=2.4 × 10
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