ArticleThe Biochemical journal1988
The pigeon heart 5'-nucleotidase responsible for ischaemia-induced adenosine formation.
Article in The Biochemical journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 69 citations in OpenAlex.
- Inborn Errors of Purine Salvage and Catabolism.Metabolites · 2023Review
- Hidden Pool of Cardiac Adenine Nucleotides That Controls Adenosine Production.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Structural Basis of the Molecular Switch between Phosphatase and Mutase Functions of Human Phosphomannomutase 1 under Ischemic Conditions.Biochemistry · 2018Article
- Evidence for control of adenosine metabolism in rat oxidative skeletal muscle by changes in pH.The Journal of physiology · 2000Article
- Structure-activity relationship of cytoplasmic 5'-nucleotidase substrate sites.The Biochemical journal · 1996Article
- Acidosis during ischemia promotes adenosine triphosphate resynthesis in postischemic rat heart. In vivo regulation of 5'-nucleotidase.The Journal of clinical investigation · 1994Article
- Effect of 5'-deoxy-5'-isobutylthioadenosine on formation and release of adenosine from neonatal and adult rat ventricular myocytes.The Biochemical journal · 1993Article
- The interaction of phospholipid bilayers with pig heart AMP deaminase: Fourier-transform infrared spectroscopic and kinetic studies.The Biochemical journal · 1993Article
- 5'-Nucleotidase: molecular structure and functional aspects.The Biochemical journal · 1992Review
- Effect of diadenosine polyphosphates on catecholamine secretion from isolated chromaffin cells.British journal of pharmacology · 1990Article
- Partial purification and properties of an AMP-specific soluble 5'-nucleotidase from pigeon heart.The Biochemical journal · 1990Article
- Immunolocalization of ecto-5'-nucleotidase in the kidney by a monoclonal antibody.Histochemistry · 1990Article
- 5'-Nucleotidases of chicken gizzard and human pancreatic adenocarcinoma cells are anchored to the plasma membrane via a phosphatidylinositol-glycan.The Biochemical journal · 1989Article
- Inhibition of IMP-specific cytosolic 5'-nucleotidase and adenosine formation in rat polymorphonuclear leucocytes by 5'-deoxy-5'-isobutylthio derivatives of adenosine and inosine.The Biochemical journal · 1989Article
- Changes in the activities of adenosine-metabolizing enzymes in six regions of the rat brain on chemical induction of hypothyroidism.The Biochemical journal · 1989Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. A 5'-nucleotidase with a strong preference for AMP over IMP was characterized in homogenates and subcellular fractions of pigeon heart by using concentrations of ATP, ADP and AMP which mimicked those present in the ischaemic tissue. 2. The AMP-5'-nucleotidase had a neutral pH optimum and an apparent Km in the range 4.6-5.2 mM. It was stimulated by ATP plus ADP, and was inhibited by other nucleoside monophosphates, Pi and p-nitrophenyl phosphate, but not by ribose 5-phosphate or beta-glycerophosphate. The enzyme was not inhibited by [alpha beta-methylene] ADP or by 5'-deoxy-5'-isobutylthioadenosine, an inhibitor of the previously purified IMP-preferring cytosolic 5'-nucleotidase. 3. Subcellular-fractionation studies indicated that the enzyme has access to cytosolic AMP, although it may be associated by weak ionic interactions with an organelle present in the low-speed particulate fraction. 4. A 5'-nucleotidase was detected under similar conditions in homogenates of rat heart. 5. The activity of the pigeon heart AMP-5'-nucleotidase was sufficient to account for previously measured rates of ischaemia-induced adenosine formation. The similar activity in rat heart could, however, account for only part of ischaemia-induced adenosine formation in this tissue.
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