ArticlePloS one2017
Biomonitoring of marine vertebrates in Monterey Bay using eDNA metabarcoding.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Getting to the Core of the Matter-Assessing the Role of Replication in Metabarcoding-Based sedaDNA.Molecular ecology resources · 2026Article
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- Environmental Filtering Weakens with Trophic Level in Urban Coastal Ecosystems.Environmental science & technology · 2026Article
- Optimization of environmental DNA-based methods: a case study for detecting brook trout (PeerJ · 2026Article
- New tools to uncover old tricks: an update on the knowledge on the most successful invasive freshwater helminth, Schyzocotyle acheilognathi.Parasitology research · 2025Review
- Multi-Tool Marine Metabarcoding Bioassessment for Baselining and Monitoring Species and Communities in Kelp Habitats.Molecular ecology resources · 2025Article
- Monitoring Fish Biodiversity in the Pelagic Zone of the Western Indian Ocean Using Environmental DNA Metabarcoding.Biology · 2025Article
- Environmental DNA illuminates the darkness of mesophotic assemblages of fishes from West Indian Ocean.PloS one · 2025Article
- Vertical and horizontal environmental DNA (eDNA) patterns of fish in a shallow and well-mixed North Sea area.Scientific reports · 2024Article
- Occurrence of recreational water quality monitoring general fecal indicator bacteria and fecal source identification genetic markers in gray seal scat.The Science of the total environment · 2024Article
- Advances in environmental DNA monitoring: standardization, automation, and emerging technologies in aquatic ecosystems.Science China. Life sciences · 2024Review
- Existing evidence on the use of environmental DNA as an operational method for studying rivers: a systematic map and thematic synthesis.Environmental evidence · 2024Article
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- Article
- Article
- Heterogeneity of Fish Taxonomic and Functional Diversity Evaluated by eDNA and Gillnet along a Mangrove-Seagrass-Coral Reef Continuum.Animals : an open access journal from MDPI · 2023Article
- Investigations of Fish Assemblages Using Two Methods in Three Terminal Reservoirs of the East Route of South-to-North Water Transfer Project, China.Animals : an open access journal from MDPI · 2023Article
- Evaluating the sampling effort for the metabarcoding-based detection of fish environmental DNA in the open ocean.Ecology and evolution · 2023Article
- Article
- Assessing a megadiverse but poorly known community of fishes in a tropical mangrove estuary through environmental DNA (eDNA) metabarcoding.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular analysis of environmental DNA (eDNA) can be used to assess vertebrate biodiversity in aquatic systems, but limited work has applied eDNA technologies to marine waters. Further, there is limited understanding of the spatial distribution of vertebrate eDNA in marine waters. Here, we use an eDNA metabarcoding approach to target and amplify a hypervariable region of the mitochondrial 12S rRNA gene to characterize vertebrate communities at 10 oceanographic stations spanning 45 km within the Monterey Bay National Marine Sanctuary (MBNMS). In this study, we collected three biological replicates of small volume water samples (1 L) at 2 depths at each of the 10 stations. We amplified fish mitochondrial DNA using a universal primer set. We obtained 5,644,299 high quality Illumina sequence reads from the environmental samples. The sequence reads were annotated to the lowest taxonomic assignment using a bioinformatics pipeline. The eDNA survey identified, to the lowest taxonomic rank, 7 families, 3 subfamilies, 10 genera, and 72 species of vertebrates at the study sites. These 92 distinct taxa come from 33 unique marine vertebrate families. We observed significantly different vertebrate community composition between sampling depths (0 m and 20/40 m deep) across all stations and significantly different communities at stations located on the continental shelf (<200 m bottom depth) versus in the deeper waters of the canyons of Monterey Bay (>200 m bottom depth). All but 1 family identified using eDNA metabarcoding is known to occur in MBNMS. The study informs the implementation of eDNA metabarcoding for vertebrate biomonitoring.
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