ArticleVirulence2017
Transcriptomic dissection of the horizontally acquired response regulator EsrB reveals its global regulatory roles in the physiological adaptation and activation of T3SS and the cognate effector repertoire in Edwardsiella piscicida during infection toward turbot.
Article in Virulence, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 63 citations in OpenAlex.
- Development and preliminary evaluation of a ΔtssB-EP as a live attenuated vaccine against Edwardsiella piscicida.Frontiers in immunology · 2026Article
- ArgR-dependent bacterial resistance to host lipid droplets in Edwardsiella piscicida.Communications biology · 2025Article
- Contrastive-learning of language embedding and biological features for cross modality encoding and effector prediction.Nature communications · 2025Article
- ChIP-seq and structural analyses delineating the regulatory mechanism of master regulator EsrB inApplied and environmental microbiology · 2024Article
- T3SS protein EsrC binds to theApplied and environmental microbiology · 2024Article
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- Transcriptome analysis ofmBio · 2024Article
- Lysine acetylation regulates the AT-rich DNA possession ability of H-NS.Nucleic acids research · 2024Article
- Type III secretion system effector YfiD inhibits the activation of host poly(ADP-ribose) polymerase-1 to promote bacterial infection.Communications biology · 2024Article
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- Applying genetic technologies to combat infectious diseases in aquaculture.Reviews in aquaculture · 2023Review
- The Attenuation Mechanism and Live Vaccine Potential of a Low-VirulenceJournal of microbiology and biotechnology · 2023Article
- Versatile lifestyles ofVirulence · 2022Article
- Xenogeneic nucleoid-associated EnrR thwarts H-NS silencing of bacterial virulence with unique DNA binding.Nucleic acids research · 2022Article
- Current status and development prospects of aquatic vaccines.Frontiers in immunology · 2022Review
- Delivering the pain: an overview of the type III secretion system with special consideration for aquatic pathogens.Veterinary research · 2021Review
- Two CD9 tetraspanin family members of Japanese flounder (Paralichthys olivaceus): characterization and comparative analysis of the anti-infectious immune function.Veterinary research · 2021Article
- Essential role of Salmonella Enteritidis DNA adenine methylase in modulating inflammasome activation.BMC microbiology · 2020Article
- Identification and study of InV as an inverse autotransporter family representative in Edwardsiella piscicida.Archives of microbiology · 2020Article
- Comparative genomics of the fish pathogensMicrobial genomics · 2020Article
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9 authors at 4 institutions in 3 countries.
Funding
Abstract
Edwardsiella piscicida is the leading pathogen threatening worldwide aquaculture industries. The 2-component system (TCS) EsrA-EsrB is essential for the pathogenesis of this bacterium. However, little is known about the regulon and regulatory mechanism of EsrA-EsrB or about the factors that mediate the interaction of TCS with bacterial hosts. Here, our RNA-seq analysis indicated that EsrB strongly induces type III and type VI secretion systems (T3/T6SS) expression and that it modulates the expression of both physiology- and virulence-associated genes in E. piscicida grown in DMEM. EsrB binds directly to a highly conserved 18-bp DNA motif to regulate the expression of T3SS and other genes. EsrB/DMEM-activated genes include 3 known and 6 novel T3SS-dependent effectors. All these effector genes are highly induced by EsrB during the late stage of in vivo infection in fish. Furthermore, although in vivo colonization by the bacterium relies on EsrB and T3/T6SS expression, it does not require the expression of individual effectors other than EseJ. The mutant lacking these 9 effectors showed significant defects in in vivo colonization and virulence toward turbot, and, more importantly, a high level of protection against challenges by wild-type E. piscicida, suggesting that it may represent a promising live attenuated vaccine. Taken together, our data demonstrate that EsrB plays a global regulatory role in controlling physiologic responses and the expression of T3SS and its cognate effector genes. Our findings will facilitate further work on the mechanism of molecular pathogenesis of this bacterium during infection.
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