ArticleScientific reports2017
Live cell imaging of mitochondria following targeted irradiation in situ reveals rapid and highly localized loss of membrane potential.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
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- Carbon ion irradiation mobilizes antitumor immunity: from concept to the clinic.Radiation oncology (London, England) · 2025Review
- How and when to measure mitochondrial inner membrane potentials.Biophysical journal · 2024Review
- Boron Nanoparticle-Enhanced Proton Therapy: Molecular Mechanisms of Tumor Cell Sensitization.Molecules (Basel, Switzerland) · 2024Article
- Cell Death, by Any Other Name….Cells · 2024Review
- Curcumin piperidone derivatives induce caspase-dependent apoptosis and suppress miRNA-21 expression in LN-18 human glioblastoma cells.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2024Article
- Targeted Central Nervous System Irradiation with Proton Microbeam Induces Mitochondrial Changes inBiology · 2023Article
- Article
- The cross-talk between Bax, Bcl2, caspases, and DNA damage in bystander HepG2 cells is regulated by γ-radiation dose and time of conditioned media transfer.Apoptosis : an international journal on programmed cell death · 2022Article
- Role of Mitochondria in Radiation Responses: Epigenetic, Metabolic, and Signaling Impacts.International journal of molecular sciences · 2021Review
- Different responses of normal cells (red blood cells) and cancer cells (K562 and K562/Dox cells) to low-doseMolecular and clinical oncology · 2021Article
- CeCILE - An Artificial Intelligence Based Cell-Detection for the Evaluation of Radiation Effects in Eucaryotic Cells.Frontiers in oncology · 2021Article
- The Small GTPase Cdc42 Negatively Regulates the Formation of Neutrophil Extracellular Traps by Engaging Mitochondria.Frontiers in immunology · 2021Article
- Radiation-Induced Immunity and Toxicities: The Versatility of the cGAS-STING Pathway.Frontiers in immunology · 2021Review
- Revisiting the Radiobiology of Targeted Alpha Therapy.Frontiers in medicine · 2021Review
- TRPV4 channel opening mediates pressure-induced pancreatitis initiated by Piezo1 activation.The Journal of clinical investigation · 2020Article
- A Multiparametric Study of Internalization of Fullerenol CInternational journal of molecular sciences · 2020Article
- Versatile magnetic microdiscs for the radio enhancement and mechanical disruption of glioblastoma cancer cells.RSC advances · 2020Article
- ATM controls DNA repair and mitochondria transfer between neighboring cells.Cell communication and signaling : CCS · 2019Article
- Monte-Carlo dosimetry and real-time imaging of targeted irradiation consequences in 2-cell stage Caenorhabditis elegans embryo.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reliance of all cell types on the mitochondrial function for survival makes mitochondria an interesting target when trying to understand their role in the cellular response to ionizing radiation. By harnessing highly focused carbon ions and protons using microbeams, we have performed in situ live cell imaging of the targeted irradiation of individual mitochondria stained with Tetramethyl rhodamine ethyl ester (TMRE), a cationic fluorophore which accumulates electrophoretically in polarized mitochondria. Targeted irradiation with both carbon ions and protons down to beam spots of <1 μm induced a near instant loss of mitochondrial TMRE fluorescence signal in the targeted area. The loss of TMRE after targeted irradiation represents a radiation induced change in mitochondrial membrane potential. This is the first time such mitochondrial responses have been documented in situ after targeted microbeam irradiation. The methods developed and the results obtained have the ability to shed new light on not just mitochondria's response to radiation but to further elucidate a putative mechanism of radiation induced depolarization and mitochondrial response.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.