ArticleJournal of industrial microbiology & biotechnology2017
Enhanced butyric acid tolerance and production by Class I heat shock protein-overproducing Clostridium tyrobutyricum ATCC 25755.
Article in Journal of industrial microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 61 citations in OpenAlex.
- Fish-Derived Clostridium butyricum LZK-08: Genomic Insights and Probiotic Evaluation in Largemouth Bass.Probiotics and antimicrobial proteins · 2026Article
- Advances in the biological production of butyric acid.World journal of microbiology & biotechnology · 2026Review
- Microbial Bio-refineries: Engineering Microbes for the Production of Versatile Short-Chain Organic Acids.Indian journal of microbiology · 2025Review
- Advancements in metabolic engineering: unlocking the potential of key organic acids for sustainable industrial applications.Frontiers in bioengineering and biotechnology · 2025Review
- Advances in stress-tolerance elements for microbial cell factories.Synthetic and systems biotechnology · 2024Review
- Gamma-Aminobutyric Acid (GABA) Biosynthesis from Lactobacillus plantarum subsp. plantarum IBRC10817 Optimized and Modeled in Response to Heat and Ultrasonic Shock.Probiotics and antimicrobial proteins · 2024Article
- Microbial Cell Factories in the Bioeconomy Era: From Discovery to Creation.Biodesign research · 2024Review
- Advance of tolerance engineering on microbes for industrial production.Synthetic and systems biotechnology · 2023Review
- Genome-Assisted Probiotic Characterization and Application ofMicroorganisms · 2023Article
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- Construction ofFrontiers in microbiology · 2023Article
- Metabolic changes of the acetogenFrontiers in microbiology · 2022Article
- Biodegradation of aromatic pollutants meets synthetic biology.Synthetic and systems biotechnology · 2021Article
- Recent advances in n-butanol and butyrate production using engineered Clostridium tyrobutyricum.World journal of microbiology & biotechnology · 2020Review
- Degeneration of industrial bacteria caused by genetic instability.World journal of microbiology & biotechnology · 2020Review
- Effects of Fructose and Overexpression of Shock-Related Gene groL on Plantaricin Q7 Production.Probiotics and antimicrobial proteins · 2020Article
- Construction of a RobustFrontiers in bioengineering and biotechnology · 2020Article
- Improving phloroglucinol tolerance and production in Escherichia coli by GroESL overexpression.Microbial cell factories · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The response of Clostridium tyrobutyricum to butyric acid stress involves various stress-related genes, and therefore overexpression of stress-related genes can improve butyric acid tolerance and yield. Class I heat shock proteins (HSPs) play an important role in the process of protecting bacteria from sudden changes of extracellular stress by assisting protein folding correctly. The results of quantitative real-time PCR indicated that the Class I HSGs grpE, dnaK, dnaJ, groEL, groES, and htpG were significantly upregulated under butyric acid stress, especially the dnaK and groE operons. Overexpression of groESL and htpG could significantly improve the tolerance of C. tyrobutyricum to butyric acid, while overexpression of dnaK and dnaJ showed negative effects on butyric acid tolerance. Acid production was also significantly promoted by increased GroESL expression levels; the final butyric acid and acetic acid concentrations were 28.2 and 38% higher for C. tyrobutyricum ATCC 25755/groESL than for the wild-type strain. In addition, when fed-batch fermentation was carried out using cell immobilization in a fibrous-bed bioreactor, the butyric acid yield produced by C. tyrobutyricum ATCC 25755/groESL reached 52.2 g/L, much higher than that for the control. The improved butyric acid yield is probably attributable to the high GroES and GroEL levels, which can stabilize the biosynthetic machinery of C. tyrobutyricum under extracellular butyric acid stress.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.