ArticleProceedings of the National Academy of Sciences of the United States of America2017
csiLSFM combines light-sheet fluorescence microscopy and coherent structured illumination for a lateral resolution below 100 nm.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 72 citations in OpenAlex.
- Aberration correction and complementary beam subtraction in light-sheet fluorescence microscopy.Journal of biomedical optics · 2026Article
- Super-Resolution Structured Illumination Microscopy to Study Endocytosis and Cellular Nanoparticle Uptake.ACS nano · 2026Review
- Photoisomerizing molecules in biological membranes.Nature reviews. Chemistry · 2026Review
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- Sound Out the Deep Clarity: Super-Resolution Photoacoustic Imaging at Depths.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2024Review
- Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging.Light, science & applications · 2024Article
- Mechanical-scan-free multicolor super-resolution imaging with diffractive spot array illumination.Nature communications · 2024Article
- Article
- Super-Resolution Imaging of Neuronal Structures with Structured Illumination Microscopy.Bioengineering (Basel, Switzerland) · 2023Article
- Superresolution structured illumination microscopy reconstruction algorithms: a review.Light, science & applications · 2023Review
- Super-resolution imaging of neuronal structure with structured illumination microscopy.bioRxiv : the preprint server for biology · 2023Article
- Doubling the resolution of light-sheet fluorescence microscopy.Nature methods · 2022Article
- Resolution doubling in light-sheet microscopy via oblique plane structured illumination.Nature methods · 2022Article
- Article
- Answering some questions about structured illumination microscopy.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2022Article
- Super-resolution microscopy: a brief history and new avenues.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2022Article
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
- High-resolution light-field microscopy with patterned illumination.Biomedical optics express · 2021Article
- GPU-accelerated real-time reconstruction in Python of three-dimensional datasets from structured illumination microscopy with hexagonal patterns.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2021Article
- Super-resolution structured illumination microscopy: past, present and future.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Light-sheet-based fluorescence microscopy (LSFM) features optical sectioning in the excitation process. It minimizes fluorophore bleaching as well as phototoxic effects and provides a true axial resolution. The detection path resembles properties of conventional fluorescence microscopy. Structured illumination microscopy (SIM) is attractive for superresolution because of its moderate excitation intensity, high acquisition speed, and compatibility with all fluorophores. We introduce SIM to LSFM because the combination pushes the lateral resolution to the physical limit of linear SIM. The instrument requires three objective lenses and relies on methods to control two counterpropagating coherent light sheets that generate excitation patterns in the focal plane of the detection lens. SIM patterns with the finest line spacing in the far field become available along multiple orientations. Flexible control of rotation, frequency, and phase shift of the perfectly modulated light sheet are demonstrated. Images of beads prove a near-isotropic lateral resolution of sub-100 nm. Images of yeast endoplasmic reticulum show that coherent structured illumination (csi) LSFM performs with physiologically relevant specimens.
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