ArticleIn vivo (Athens, Greece)
Functional Roles of Netrin-1 in Osteoblast Differentiation.
Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
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- Netrin1 patterns the dorsal spinal cord through modulation of Bmp signaling.Cell reports · 2024Article
- Craniofacial dysmorphology in Down syndrome is caused by increased dosage of Dyrk1a and at least three other genes.Development (Cambridge, England) · 2023Article
- Overexpression of Neurogenin 1 Negatively Regulates Osteoclast and Osteoblast Differentiation.International journal of molecular sciences · 2022Article
- Article
- Characteristics of sensory innervation in synovium of rats within different knee osteoarthritis models and the correlation between synovial fibrosis and hyperalgesia.Journal of advanced research · 2022Article
- Role of Slit/Robo Signaling pathway in Bone Metabolism.International journal of biological sciences · 2022Review
- Netrin-1 functions as a suppressor of bone morphogenetic protein (BMP) signaling.Scientific reports · 2021Article
- Crosstalk between Bone and Nerves within Bone.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Molecular patterning of the embryonic cranial mesenchyme revealed by genome-wide transcriptional profiling.Developmental biology · 2019Article
- A network map of netrin receptor UNC5B-mediated signaling.Journal of cell communication and signaling · 2019Article
- Does osteogenic potential of clonal human bone marrow mesenchymal stem/stromal cells correlate with their vascular supportive ability?Stem cell research & therapy · 2018Article
- Contact Co-culture of Osteoblasts and Sympathetic Neuronal Cells Promotes Differentiation of Both Cell Types.In vivo (Athens, Greece)Article
- Netrin-4 Promotes Differentiation and Migration of Osteoblasts.In vivo (Athens, Greece)Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimRecent studies have demonstrated that netrin-1 plays a vital role in bone metabolism. Previous studies have shown that osteoblasts produce netrin-1 which affects osteoclast differentiation. However, the role of netrin-1 in osteoblast differentiation is not well understood. In this study, we explored the roles of netrin-1 in osteoblasts. MATERIALS AND
methodsQuantitative reverse-transcriptase polymerase chain reaction (qPCR), RNA interference for netrin receptors, the generation of netrin-1 plasmid, transfection of plasmids, and cell proliferation assay were performed.
resultsDuring osteoblast differentiation by ascorbic acid, netrin-1 expression was significantly decreased. Gene expression related with osteoblast differentiation was down-regulated by netrin-1 treatment. We also found that osteoblast differentiation by bone morphogenetic protein-4 (BMP-4) was inhibited in the presence of recombinant netrin-1. Forced expression of both BMP-4 and netrin-1 significantly decreased alkaline phosphatase expression. On the other hand, Unc5b, neogenin, and A2b which belong to netrin receptors were expressed by osteoblasts. Moreover, alkaline phosphatase expression was significantly decreased by knockdown for the combination of two receptors among these receptors.
conclusionNetrin-1 is involved in the regulation of osteoblast differentiation.
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