Evidence map›Paper›PMID 28438567›Full record

ArticleBiochemical pharmacology2017

A systematic evaluation of microRNAs in regulating human hepatic CYP2E1.

Yong Wang, Dianke Yu, William H Tolleson, Li-Rong Yu, Bridgett Green, Linjuan Zeng, Yinting Chen, Si Chen, Zhen Ren, Lei Guo and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical pharmacology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
3.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 40 citations in OpenAlex.

  1. DICER-driven regulation of lipid metabolism and macrophage-adipocyte crosstalk in partial lipodystrophy.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2026
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  5. Novel Approaches to Characterize Individual Drug Metabolism and Advance Precision Medicine.Drug metabolism and disposition: the biological fate of chemicals · 2023
    Review
  6. Review
  7. Article
  8. Review
  9. Article
  10. Review
  11. Review
  12. miR-15a-3p Protects Against Isoniazid-Induced Liver InjuryFrontiers in molecular biosciences · 2021
    Article
  13. Article
  14. Review
  15. Review
  16. Review
  17. Review
  18. Article
  19. Porcine cytochrome 2A19 and 2E1.Basic & clinical pharmacology & toxicology · 2019
    Review
  20. Regulation of cytochrome P450 expression by microRNAs and long noncoding RNAs: Epigenetic mechanisms in environmental toxicology and carcinogenesis.Journal of environmental science and health. Part C, Environmental carcinogenesis & ecotoxicology reviews · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Yong WangEye Institute, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China; National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
Dianke YuNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
William H TollesonNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
Li-Rong YuNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
Bridgett GreenNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
Linjuan ZengNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
Yinting ChenNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
Si ChenNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
Zhen RenNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
Lei GuoNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
Weida TongNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
Huaijin GuanEye Institute, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China. Electronic address: huaijinguaneye@163.com.
Baitang NingNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA. Electronic address: baitang.ning@fda.hhs.gov.
United States Food and Drug Administration · USNational Center for Toxicological Research · USAffiliated Hospital of Nantong University · CN

Funding

Intramural FDA HHS FD999999
6 · The paper itself

Abstract

Cytochrome P450 2E1 (CYP2E1) is an important drug metabolizing enzyme for processing numerous xenobiotics in the liver, including acetaminophen and ethanol. Previous studies have shown that microRNAs (miRNAs) can suppress CYP2E1 expression by binding to the 3'-untranslated region (3'-UTR) of its transcript. However, a systematic analysis of CYP2E1 regulation by miRNAs has not been described. Here, we applied in silico, in vivo, and in vitro approaches to investigate miRNAs involved in the regulation of CYP2E1. Initially, potential miRNA binding sites in the CYP2E1 mRNA transcript were identified and screened using in silico methods. Next, inverse correlations were found in human liver samples between the expression of CYP2E1 mRNA and the levels of two miRNA species, hsa-miR-214-3p and hsa-miR-942-5p. In a HepG2-derived CYP2E1 over-expression cell model, hsa-miR-214-3p exhibited strong suppression of CYP2E1 expression by targeting the coding region of its mRNA transcript, but hsa-miR-942-5p did not inhibit CYP2E1 levels. Electrophoretic mobility shift assays confirmed that hsa-miR-214-3p recruited other cellular protein factors to form stable complexes with specific sequences present in the CYP2E1 mRNA open reading frame. Transfection of HepaRG cells with hsa-miR-214-3p mimics inhibited expression of the endogenous CYP2E1 gene. Further, hsa-miR-214-3p mimics partially blocked ethanol-dependent increases in CYP2E1 mRNA and protein levels in HepG2 cells and they reduced the release of alanine aminotransferase from CYP2E1-overexpressing HepG2 cells exposed to acetaminophen. These results substantiate the suppressing effect of hsa-miR-214-3p on CYP2E1 expression.

Indexed as

Gene Expression Regulation, EnzymologicModels, BiologicalAcetaminophenAlanine TransaminaseAnalgesics, Non-NarcoticBinding SitesBiomarkersComputational BiologyCytochrome P-450 CYP2E1Databases, Nucleic AcidElectrophoretic Mobility Shift AssayExpert SystemsHepatocytesHep G2 CellsHumansMicroRNAsAcetaminophenAlanine TransaminaseAnalgesics, Non-NarcoticBiomarkersCytochrome P-450 CYP2E1MicroRNAsMIRN214 microRNA, humanMIRN942 microRNA, humanRNARNA, MessengerRNA, recombinantCYP2E1Drug metabolizing enzymesInter-individual variabilitymicroRNA, hsa-miR-214-3pPharmacogenomics

Identifiers

PMID28438567
PMCPMC5703043
OpenAlexW2607106343

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.