ArticleMicrobial cell factories2017
Expression of the Clonostachys rosea lactonohydrolase gene by Lactobacillus reuteri to increase its zearalenone-removing ability.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 54 citations in OpenAlex.
- Isolation and Characterization of Sphingopyxis sp. Q841: A New Zearalenone- Degrading Strain.Current microbiology · 2026Article
- Genetically Modified Lactic Acid Bacteria in the EU Food Chain: Applications, Benefits, and Risk Assessment.International journal of molecular sciences · 2026Review
- Isolation and characterization of a novel strain of Rhizopus arrhizus for effective biodegradation and detoxification of zearalenone.World journal of microbiology & biotechnology · 2026Article
- Zearalenone causes female reproductive lipotoxicity through the ERα-CD36/TLR4 signaling pathway.Communications biology · 2026Article
- A universal medium bridging the shake-flask to fermenter gap inFrontiers in microbiology · 2026Article
- Engineering Escherichia coli nissle 1917 with lactonase ZHD101 alleviates zearalenone-induced intestinal disruption and reproductive toxicity in rat.World journal of microbiology & biotechnology · 2025Article
- Advances in zearalenone-degrading enzymes research: characteristics, mining, improvement, and application.Journal of animal science and biotechnology · 2025Review
- Biodegradation of Zearalenone by a NovelMicroorganisms · 2025Article
- Genomic and phenotypic characterization of two novel human-derivedFrontiers in microbiology · 2025Article
- Bacterial Lactonases ZenA with Noncanonical Structural Features Hydrolyze the Mycotoxin Zearalenone.ACS catalysis · 2024Article
- Mechanisms by which microbial enzymes degrade four mycotoxins and application in animal production: A review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Review
- Mutation, food-grade expression, and characterization of a lactonase for zearalenone degradation.Applied microbiology and biotechnology · 2023Article
- Induced Expression of theToxins · 2023Article
- Post-Harvest Prevention of Fusariotoxin Contamination of Agricultural Products by Irreversible Microbial Biotransformation: Current Status and Prospects.Biotech (Basel (Switzerland)) · 2023Review
- Review
- Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil.Toxins · 2022Article
- Crosstalk between Mycotoxins and Intestinal Microbiota and the Alleviation Approach via Microorganisms.Toxins · 2022Review
- Toxicity and preventive approaches of Fusarium derived mycotoxins using lactic acid bacteria: state of the art.Biotechnology letters · 2022Review
- Cloning and Characterization of Three Novel Enzymes Responsible for the Detoxification of Zearalenone.Toxins · 2022Article
- Biological Detoxification of Mycotoxins: Current Status and Future Advances.International journal of molecular sciences · 2022Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMycotoxins are secondary metabolites produced by filamentous fungi that can contaminate agricultural crops in the field as well as during harvest, transportation, processing, or storage. Zearalenone (ZEN), a non-steroidal estrogenic mycotoxin, produced by Fusarium species, has been shown to be associated with reproductive disorders in farm animals and to a lesser extent in hyperoestrogenic syndromes in humans. Thus, the decontamination of ZEN in foods and feeds is an important issue.
resultsIn this study, the gene encoding ZHD101, a ZEN-degrading enzyme produced by Clonostachys rosea IFO 7063, was cloned into an Escherichia coli-Lactobacillus shuttle vector, pNZ3004, and the resultant plasmid pNZ-zhd101 was then introduced via electroporation into Lactobacillus reuteri Pg4, a probiotic strain isolated from the gastrointestinal tract of broilers. The transformed strain L. reuteri pNZ-zhd101 acquired the capacity to degrade ZEN. In addition, the production of recombinant ZHD101 did not affect cell growth, acid and bile salt tolerance, and had only a minor effect on the adhesion ability of L. reuteri pNZ-zhd101.
conclusionsTo the best of our knowledge, this is the first report of successful expression of a ZEN-degrading enzyme by intestinal lactobacilli.
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