ArticleReproductive biology and endocrinology : RB&E2017
Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device.
Article in Reproductive biology and endocrinology : RB&E, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Modification of cryoprotectants on sperm cryopreservation: A study of embryo development.Journal of advanced veterinary and animal research · 2024Article
- Alterations in Gene Expression and the Fatty Acid Profile Impact but Do Not Compromise the In Vitro Maturation of Zebrafish (Animals : an open access journal from MDPI · 2023Article
- An all-37 °C thawing method improves the clinical outcomes of vitrified frozen-thawed embryo transfer: a retrospective study using a case-control matching analysis.Archives of gynecology and obstetrics · 2023Article
- A comprehensive review and update on human fertility cryopreservation methods and tools.Frontiers in veterinary science · 2023Review
- A human-based assisted reproduction protocol for the menstruating spiny mouse, Acomys cahirinus.PloS one · 2020Article
- Cryodevices developed for minimum volume cooling vitrification of bovine oocytes.Animal science journal = Nihon chikusan GakkaihoReview
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Authors and funding
8 authors.
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Abstract
backgroundCurrently, the cryopreservation of embryos and oocytes is essential for assisted reproductive technology (ART) laboratories worldwide. This study aimed to evaluate the efficacy of the Kitasato Vitrification System (KVS) as a vitrification device for the cryopreservation of mouse embryos to determine whether this novel device can be adapted to the field of ART.
methodsIn Experiment 1, blastocysts were vitrified using the KVS. Vitrified blastocysts were warmed and subsequently cultured for 72 h. In Experiment 2, 2-cell-stage embryos were vitrified using the KVS, and vitrified embryos were warmed and subsequently cultured for 96 h. In Experiment 3, we evaluated the in vivo developmental potential of vitrified 2-cell-stage embryos using the KVS, and in Experiment 4, we evaluated the cooling and warming rates for these devices using a numerical simulation.
resultsIn Experiment 1, there were no significant differences between the survival rates of the KVS and a control device. However, re-expanded (100%) and hatching (91.8%) rates were significantly higher for blastocysts vitrified using the KVS. In Experiment 2, there were no significant differences between the survival rates, or rates of development to the blastocyst stage, of vitrified and fresh embryos. In Experiment 3, after embryo transfer, 41% of the embryos developed into live offspring. In Experiment 4, the cooling and warming rates of the KVS were 683,000 and 612,000 °C/min, respectively, exceeding those of the control device.
conclusionsOur study clearly demonstrates that the KVS is a novel vitrification device for the cryopreservation of mouse embryos at the blastocyst and 2-cell stage.
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