ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2017
Tyramide Signal Amplification Permits Immunohistochemical Analyses of Androgen Receptors in the Rat Prefrontal Cortex.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Comparison of Cerebral Blood Flow in Regions Relevant to Cognition After Enzalutamide, Darolutamide, and Placebo in Healthy Volunteers: A Randomized Crossover Trial.Targeted oncology · 2023Trial
- Abnormal alterations in neurodevelopment in preterm children with very low birth weight during the adolescence.European child & adolescent psychiatry · 2025Article
- Exploring Multiplex Immunohistochemistry (mIHC) Techniques and Histopathology Image Analysis: Current Practice and Potential for Clinical Incorporation.Cancer medicine · 2025Review
- Associations Among Birth Weight, Adrenarche, Brain Morphometry, and Cognitive Function in Preterm Children Ages 9 to 11 Years.Biological psychiatry. Cognitive neuroscience and neuroimaging · 2024Article
- Neurosteroids and the mesocorticolimbic system.Neuroscience and biobehavioral reviews · 2023Review
- Neurodevelopmental Model Explaining Associations between Sex Hormones, Personality, and Eating Pathology.Brain sciences · 2023Review
- Deletion of Androgen Receptor in LepRb Cells Improves Estrous Cycles in Prenatally Androgenized Mice.Endocrinology · 2023Article
- Distribution of androgen receptor mRNA in the prepubertal male and female mouse brain.Journal of neuroendocrinology · 2021Article
- Sex differences in dopamine innervation and microglia are altered by synthetic progestin in neonatal medial prefrontal cortex.Journal of neuroendocrinology · 2021Article
- Effects of Biological Sex and Stress Exposure on Ventromedial Prefrontal Regulation of Mood-Related Behaviors.Frontiers in behavioral neuroscience · 2021Review
- ERα Signaling in GHRH/Kiss1 Dual-Phenotype Neurons Plays Sex-Specific Roles in Growth and Puberty.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Lack of AR in LepRb Cells Disrupts Ambulatory Activity and Neuroendocrine Axes in a Sex-Specific Manner in Mice.Endocrinology · 2020Article
- Editorial: Steroids and the Brain.Frontiers in endocrinology · 2020Article
- Androgen Regulation of the Mesocorticolimbic System and Executive Function.Frontiers in endocrinology · 2018Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Research on neural androgen receptors (ARs) has traditionally focused on brain regions that regulate reproductive and aggressive behaviors, such as the hypothalamus and amygdala. Although many cells in the prefrontal cortex (PFC) also express ARs, the number of ARs per cell appears to be much lower, and thus, AR immunostaining is often hard to detect and quantify in the PFC. Here, we demonstrate that biotin tyramide signal amplification (TSA) dramatically increases AR immunoreactivity in the rat brain, including critical regions of the PFC such as the medial PFC (mPFC) and orbitofrontal cortex (OFC). We show that TSA is useful for AR detection with both chromogenic and immunofluorescent immunohistochemistry. Double-labeling studies reveal that AR+ cells in the PFC and hippocampus are NeuN+ but not GFAP+ and thus primarily neuronal. Finally, in gonadally intact rats, more AR+ cells are present in the mPFC and OFC of males than of females. Future studies can use TSA to further examine AR immunoreactivity across ages, sexes, strains, and different procedures (e.g., fixation methods). In light of emerging evidence for the androgen regulation of executive function and working memory, these results may help understand the distribution and roles of ARs in the PFC.
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