Evidence map›Paper›PMID 28438092›Full record

ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2017

Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands.

Laura Gómez-Santos, Edurne Alonso, Lucio Díaz-Flores, Juan F Madrid, Francisco J Sáez

Open access · bronzeAbstract read
In one paragraph

Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 6 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Laura Gómez-SantosDepartment of Cell Biology and Histology, Training and Research Unit: Reproduction, Development, Aging and Cancer (TRU/UFI 11/44), School of Medicine and Nursing, University of the Basque Country UPV/EHU, Leioa, Vizcaya, Spain (LG-S, EA, FJS).
Edurne AlonsoDepartment of Cell Biology and Histology, Training and Research Unit: Reproduction, Development, Aging and Cancer (TRU/UFI 11/44), School of Medicine and Nursing, University of the Basque Country UPV/EHU, Leioa, Vizcaya, Spain (LG-S, EA, FJS).
Lucio Díaz-FloresDepartment of Anatomy, Pathology, Histology and Radiology, University of La Laguna, San Cristóbal de La Laguna, Tenerife, Spain (LD-F).
Juan F MadridDepartment of Cell Biology and Histology, Regional Campus of International Excellence "Campus Mare Nostrum," IMIB-Arrixaca, School of Medicine, University of Murcia, Espinardo, Murcia, Spain (JFM).
Francisco J SáezDepartment of Cell Biology and Histology, Training and Research Unit: Reproduction, Development, Aging and Cancer (TRU/UFI 11/44), School of Medicine and Nursing, University of the Basque Country UPV/EHU, Leioa, Vizcaya, Spain (LG-S, EA, FJS).
Universidad de La Laguna · ESUniversidad de Murcia · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Parietal cells undergo a differentiation process while they move from the isthmus toward the pits and the base region of the gastric gland. The aim of this work was to analyze the rat gastric glands by lectin histochemistry to show the glycans expressed by upper (young) and lower (old) parietal cells. We used lectins recognizing the most frequent sugar moieties in mammals. Each lectin was assayed alone and in combination with several deglycosylation pretreatments: (1) β-elimination, which removes O-linked oligosaccharides; (2) incubation with Peptide-N-glycosidase F, to remove N-linked glycans; (3) acid hydrolysis, which removes terminal sialic acid moieties; (4) methylation-saponification, to remove sulfate groups from sugar residues; and (5) glucose oxidase, a technique carried out with the lectin concanavalin A to convert glucose into gluconic acid. The lectins from Helix pomatia, Dolichos biflorus (DBA), Glycine max (soybean), Maclura pomifera, Arachis hypogaea (peanut), Bandeiraea simplicifolia (lectin I-B

Indexed as

AnimalsHistocytochemistryHydrolysisMaleOligosaccharidesParietal Cells, GastricPlant LectinsPolysaccharidesRats, Sprague-DawleyOligosaccharidesPlant LectinsPolysaccharidesdifferentiationglandular stomachglycoconjugateslectinoligosaccharidesstem cells

Identifiers

PMID28438092
PMCPMC5407534
OpenAlexW2591078002

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.