ArticlePloS one2017
Infective respiratory syncytial virus is present in human cord blood samples and most prevalent during winter months.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Collateral Damage in the Placenta during Viral Infection in Pregnancy: A Possible Mechanism for Vertical Transmission and an Adverse Pregnancy Outcome.Diseases (Basel, Switzerland) · 2024Review
- Prenatal Infection by Respiratory Viruses Is Associated with Immunoinflammatory Responses in the Fetus.American journal of respiratory and critical care medicine · 2024Article
- Prenatal exposure to RSV season influences first-year risk of RSV lower respiratory illness and RSV-specific immune responses assessed at birth.Journal of virology · 2023Article
- Article
- Effects of Vertical Transmission of Respiratory Viruses to the Offspring.Frontiers in immunology · 2022Review
- Respiratory syncytial virus seropositivity at birth is associated with adverse neonatal respiratory outcomes.Pediatric pulmonology · 2020Article
- Evaluation of Droplet Digital Polymerase Chain Reaction (ddPCR) for the Absolute Quantification ofInternational journal of molecular sciences · 2020Article
- Effects of maternal-fetal transmission of viruses and other environmental agents on lung development.Pediatric research · 2020Review
- The Burden of Respiratory Syncytial Virus Infection in Adults and Reproductive-Aged Women.Global health, science and practice · 2019Article
- The BeWo cell line derived from a human placental choriocarcinoma is permissive for respiratory syncytial virus infection.Virus genes · 2019Article
- Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman respiratory syncytial virus (RSV) remains the most common cause of severe lower respiratory tract disease amongst infants, and continues to cause annual epidemics of respiratory disease every winter worldwide. Demonstrating placental transmission of viable RSV in human samples is a major paradigm shift in respiratory routes considered likely for RSV transmission.
methodsDroplet digital PCR (ddPCR) was used to identify RSV present in cord blood mononucleocytes (CBM). CBMs testing positive for RSV were treated with phytohemagglutinin (PHA), PHA and nitric oxide (NO) or PHA, NO and palivizumab, and co-cultured with HeLa cell monolayers. Subsequent immuno-staining for RSV was used to visualize infective viral plaques.
resultsRSV was detected in 26 of 45 samples (57.7%) by ddPCR. CBM's collected in winter were more likely to test positive for RSV (17/21 samples, risk = 80%, OR = 7.08; 95% CI 1.80-27.80; p = 0.005) compared to non-winter months (9/24 samples, 37.5%). RSV plaques were observed in non-treated and treated co-cultured HeLa monolayers.
conclusionsDemonstrating active RSV in CBMs suggests in utero transmission of infective virus to the fetus without causing overt disease. This is likely to have an important impact on immune development as well as future virus-host interactions, thereby warranting further investigation.
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