ArticleJournal of proteome research2017
HILAQ: A Novel Strategy for Newly Synthesized Protein Quantification.
Article in Journal of proteome research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
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- Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts:Chemical reviews · 2025Review
- Time-resolved interactome profiling deconvolutes secretory protein quality control dynamics.Molecular systems biology · 2024Article
- Nascent Proteome and Glycoproteome Reveal the Inhibition Role of ALG1 in Hepatocellular Carcinoma Cell Migration.Phenomics (Cham, Switzerland) · 2022Article
- Extracellular matrix dynamics: tracking in biological systems and their implications.Journal of biological engineering · 2022Review
- Protein synthesis control in cancer: selectivity and therapeutic targeting.The EMBO journal · 2022Review
- Recent advancements in mass spectrometry-based tools to investigate newly synthesized proteins.Current opinion in chemical biology · 2022Review
- Nascent Glycoproteome Reveals That N-Linked Glycosylation Inhibitor-1 Suppresses Expression of Glycosylated Lysosome-Associated Membrane Protein-2.Frontiers in molecular biosciences · 2022Article
- Temporal Quantitative Profiling of Newly Synthesized Proteins during Aβ Accumulation.Journal of proteome research · 2021Article
- Global analysis of protein degradation in prion infected cells.Scientific reports · 2020Article
- Proteomic Techniques to Examine Neuronal Translational Dynamics.International journal of molecular sciences · 2019Review
- Recent technical advances in proteomics.F1000Research · 2019Review
- A Proteomics Approach to Profiling the Temporal Translational Response to Stress and Growth.iScience · 2018Article
- Chemical proteomics reveals new targets of cysteine sulfinic acid reductase.Nature chemical biology · 2018Article
- Quantitative analysis of newly synthesized proteins.Nature protocols · 2018Article
- Proteomics and pulse azidohomoalanine labeling of newly synthesized proteins: what are the potential applications?Expert review of proteomics · 2018Review
- Methods for monitoring and measurement of protein translation in time and space.Molecular bioSystems · 2017Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Here we describe a new strategy, HILAQ (Heavy Isotope Labeled Azidohomoalanine Quantification), to rapidly quantify the molecular vulnerability profile to oxytosis, which is an oxidative stress-induced programed cell death pathway that has been reported to be involved in aging and neurodegenerative diseases. HILAQ was able to quantify 1962 newly synthesized proteins (NSPs) after 1 h of pulse labeling in HEK293T cell line, while 353 proteins were quantified using the previously published QuaNCAT protocol. HILAQ was successfully applied to the HT22 oxytosis model. 226 proteins were found to have a two-fold change in abundance, and 108 proteins were enriched in the cell death pathway, demonstrating the utility of HT22 cells as a tool to study the molecular details of cell death involved in neurodegenerative diseases. The HILAQ strategy simplifies the analysis of newly synthesized proteomes through the use of isobaric labels and achieves higher sensitivity than previously published methods.
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