ArticleCellular microbiology2017
A novel dense granule protein, GRA41, regulates timing of egress and calcium sensitivity in Toxoplasma gondii.
Article in Cellular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- TGIF is a Golgin-like protein required for Golgi structural maintenance and function inbioRxiv : the preprint server for biology · 2026Article
- Molecular interactions ofFrontiers in veterinary science · 2025Article
- Functional dissection of prenyltransferases reveals roles in endocytosis and secretory vacuolar sorting in type 2-ME49 strain ofVirulence · 2024Article
- Egress Regulatory Factors: HowPathogens (Basel, Switzerland) · 2023Review
- CRISPR Screens IdentifymBio · 2023Article
- Functional Characterization of 15 Novel Dense Granule Proteins in Toxoplasma gondii Using the CRISPR-Cas9 System.Microbiology spectrum · 2023Article
- Down the membrane hole: Ion channels in protozoan parasites.PLoS pathogens · 2022Review
- A positive feedback loop mediates crosstalk between calcium, cyclic nucleotide and lipid signalling in calcium-induced Toxoplasma gondii egress.PLoS pathogens · 2022Article
- The Tyrosine Phosphatase PRL Regulates Attachment of Toxoplasma gondii to Host Cells and Is Essential for Virulence.mSphere · 2022Article
- The Dually Localized EF-Hand Domain-Containing Protein TgEFP1 Regulates the Lytic Cycle ofCells · 2022Article
- Ferlins and TgDOC2 inLife (Basel, Switzerland) · 2021Article
- Effect of deletion of gra17 and gra23 genes on the growth, virulence, and immunogenicity of type II Toxoplasma gondii.Parasitology research · 2020Article
- Article
- A plasma membrane localized protein phosphatase in Toxoplasma gondii, PPM5C, regulates attachment to host cells.Scientific reports · 2019Article
- Phosphatidic acid governs natural egress in Toxoplasma gondii via a guanylate cyclase receptor platform.Nature microbiology · 2019Article
- TgDrpC, an atypical dynamin-related protein in Toxoplasma gondii, is associated with vesicular transport factors and parasite division.Molecular microbiology · 2019Article
- Calcium signaling and the lytic cycle of the Apicomplexan parasite Toxoplasma gondii.Biochimica et biophysica acta. Molecular cell research · 2018Review
- A Window toPathogens (Basel, Switzerland) · 2018Review
- Functional Characterization of Dense Granule Proteins inFrontiers in cellular and infection microbiology · 2018Article
- Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Toxoplasma gondii is an obligate intracellular apicomplexan parasite with high seroprevalence in humans. Repeated lytic cycles of invasion, replication, and egress drive both the propagation and the virulence of this parasite. Key steps in this cycle, including invasion and egress, depend on tightly regulated calcium fluxes and, although many of the calcium-dependent effectors have been identified, the factors that detect and regulate the calcium fluxes are mostly unknown. To address this knowledge gap, we used a forward genetic approach to isolate mutants resistant to extracellular exposure to the calcium ionophore A23187. Through whole genome sequencing and complementation, we have determined that a nonsense mutation in a previously uncharacterised protein is responsible for the ionophore resistance of one of the mutants. The complete loss of this protein recapitulates the resistance phenotype and importantly shows defects in calcium regulation and in the timing of egress. The affected protein, GRA41, localises to the dense granules and is secreted into the parasitophorous vacuole where it associates with the tubulovesicular network. Our findings support a connection between the tubulovesicular network and ion homeostasis within the parasite, and thus a novel role for the vacuole of this important pathogen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.