ArticleScience advances2017
Label-free optical detection of single enzyme-reactant reactions and associated conformational changes.
Article in Science advances, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
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- Single-atomic-ion detection with plasmon-enhanced whispering-gallery-mode microlasers.Nature photonics · 2026Article
- Plasmonic biosensors and actuators for integrated point-of-care diagnostics.Npj biosensing · 2025Review
- Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
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- High-Q WGM microcavity-based optofluidic sensor technologies for biological analysis.Biomicrofluidics · 2024Review
- Whispering-Gallery Mode Optoplasmonic Microcavities: From Advanced Single-Molecule Sensors and Microlasers to Applications in Synthetic Biology.ACS photonics · 2024Review
- Emerging nanophotonic biosensor technologies for virus detection.Nanophotonics (Berlin, Germany) · 2022Review
- Demonstration of intracellular real-time molecular quantification via FRET-enhanced optical microcavity.Nature communications · 2022Article
- Ceruloplasmin in flatland: the relationship between enzyme catalytic activity and surface hydrophilicity.RSC advances · 2022Article
- Label-Free Imaging of Single Proteins and Binding Kinetics Using Total Internal Reflection-Based Evanescent Scattering Microscopy.Analytical chemistry · 2022Article
- Controlled nano-agglomerates as stabile SERS reporters for unequivocal labelling.Scientific reports · 2022Article
- Modelling of the dynamic polarizability of macromolecules for single-molecule optical biosensing.Scientific reports · 2022Article
- Nanosecond time scale transient optoplasmonic detection of single proteins.Science advances · 2022Article
- Photothermal Spectro-Microscopy as Benchmark for Optoplasmonic Bio-Detection Assays.The journal of physical chemistry. C, Nanomaterials and interfaces · 2021Article
- Nanophotonic biosensors harnessing van der Waals materials.Nature communications · 2021Review
- Sensing Enzyme Activation Heat Capacity at the Single-Molecule Level Using Gold-Nanorod-Based Optical Whispering Gallery Modes.ACS applied nano materials · 2021Article
- Whispering-gallery microlasers for cell tagging and barcoding: the prospects for in vivo biosensing.Light, science & applications · 2021Article
- 1/f-noise-free optical sensing with an integrated heterodyne interferometer.Nature communications · 2021Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monitoring the kinetics and conformational dynamics of single enzymes is crucial to better understand their biological functions because these motions and structural dynamics are usually unsynchronized among the molecules. However, detecting the enzyme-reactant interactions and associated conformational changes of the enzyme on a single-molecule basis remains as a challenge to established optical techniques because of the commonly required labeling of the reactants or the enzyme itself. The labeling process is usually nontrivial, and the labels themselves might skew the physical properties of the enzyme. We demonstrate an optical, label-free method capable of observing enzymatic interactions and associated conformational changes on a single-molecule level. We monitor polymerase/DNA interactions via the strong near-field enhancement provided by plasmonic nanorods resonantly coupled to whispering gallery modes in microcavities. Specifically, we use two different recognition schemes: one in which the kinetics of polymerase/DNA interactions are probed in the vicinity of DNA-functionalized nanorods, and the other in which these interactions are probed via the magnitude of conformational changes in the polymerase molecules immobilized on nanorods. In both approaches, we find that low and high polymerase activities can be clearly discerned through their characteristic signal amplitude and signal length distributions. Furthermore, the thermodynamic study of the monitored interactions suggests the occurrence of DNA polymerization. This work constitutes a proof-of-concept study of enzymatic activities using plasmonically enhanced microcavities and establishes an alternative and label-free method capable of investigating structural changes in single molecules.
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