ArticleTheranostics2017
Physiological Effects of Ac4ManNAz and Optimization of Metabolic Labeling for Cell Tracking.
Article in Theranostics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
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- Mapping GlycoRNAs on an Exosomal Surface.Journal of the American Chemical Society · 2026Article
- Beyond labeling: differential AcRegenerative biomaterials · 2026Article
- Novel Strategy for Acquiring Metabolically-Tagged Nascent Extracellular Vesicles: Implications for Identifying Surface Protein Markers of Extracellular Vesicles From Neuroblastoma Cells Cultured With Native Serum.Journal of extracellular vesicles · 2025Article
- Optimization of glycopeptide enrichment techniques for the identification of clinical biomarkers.Expert review of proteomics · 2024Review
- Engineered Cell Membrane-Camouflaged Nanomaterials for Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2024Review
- Profiling specific cell populations within the inflammatory tumor microenvironment by oscillating-gradient diffusion-weighted MRI.Journal for immunotherapy of cancer · 2023Article
- Spinal cord tissue engineering via covalent interaction between biomaterials and cells.Science advances · 2023Article
- From Bench to Cell: A Roadmap for Assessing the Bioorthogonal "Click" Reactivity of Magnetic Nanoparticles for Cell Surface Engineering.Bioconjugate chemistry · 2022Article
- Dual-aptamer-engineered M1 macrophage with enhanced specific targeting and checkpoint blocking for solid-tumor immunotherapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- Generation of triacyl lipopeptide-modified glycoproteins by metabolic glycoengineering as the neoantigen to boost anti-tumor immune response.Theranostics · 2021Article
- Exploring the Potential of β-CateninMolecules (Basel, Switzerland) · 2020Article
- Effect of Cell Labeling on the Function of Human Pluripotent Stem Cell-Derived Cardiomyocytes.International journal of stem cells · 2020Article
- Recent Advances in Glycoproteomic Analysis by Mass Spectrometry.Analytical chemistry · 2020Review
- Optimization and Stability of Cell-Polymer Hybrids Obtained by "Clicking" Synthetic Polymers to Metabolically Labeled Cell Surface Glycans.Biomacromolecules · 2019Article
- Click Chemistry as a Tool for Cell Engineering and Drug Delivery.Molecules (Basel, Switzerland) · 2019Review
- Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for Tracking.Scientific reports · 2018Article
- Facile metabolic glycan labeling strategy for exosome tracking.Biochimica et biophysica acta. General subjects · 2018Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic labeling techniques are powerful tools for cell labeling, tracking and proteomic analysis. However, at present, the effects of the metabolic labeling agents on cell metabolism and physiology are not known. To address this question, in this study, we analyzed the effects of cells treated with Ac4ManNAz through microarray analysis and analyses of membrane channel activity, individual bio-physiological properties, and glycolytic flux. According to the results, treatment with 50 μM Ac4ManNAz led to the reduction of major cellular functions, including energy generation capacity, cellular infiltration ability and channel activity. Interestingly, 10 μM Ac4ManNAz showed the least effect on cellular systems and had a sufficient labeling efficiency for cell labeling, tracking and proteomic analysis. Based on our results, we suggest 10 μM as the optimum concentration of Ac4ManNAz for
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