Evidence map›Paper›PMID 28435049›Full record

ArticleMolecular and cellular endocrinology2017

Exposure of decidualized HIESC to low oxygen tension and leucine deprivation results in increased IGFBP-1 phosphorylation and reduced IGF-I bioactivity.

Majida Abu Shehab, Kyle Biggar, Sahil Sagar Singal, Karen Nygard, Shawn Shun-Cheng Li, Thomas Jansson, Madhulika B Gupta

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular endocrinology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Majida Abu ShehabChildren's Health Research Institute, University of Western Ontario, London, Ontario, Canada.
Kyle BiggarDepartment of Biochemistry, University of Western Ontario, London, Ontario, Canada.
Sahil Sagar SingalDepartment of Biochemistry, University of Western Ontario, London, Ontario, Canada.
Karen NygardDepartment of Biotron, University of Western Ontario, London, Ontario, Canada.
Shawn Shun-Cheng LiDepartment of Biochemistry, University of Western Ontario, London, Ontario, Canada.
Thomas JanssonDepartment of Obstetrics & Gynecology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Madhulika B GuptaChildren's Health Research Institute, University of Western Ontario, London, Ontario, Canada; Department of Biochemistry, University of Western Ontario, London, Ontario, Canada; Department of Pediatrics, University of Western Ontario, London, Ontario, Canada. Electronic address: mbgupta@uwo.ca.
Western University · CAChildren’s Health Research Institute · CAUniversity of Colorado Anschutz Medical Campus · US

Funding

PILOT STUDY--SUBSTRATE METABOLISM IN EXTREMELY LOW BIRTH WEIGHT INFANTSP30DK048520 · NIDDK · UNIVERSITY OF COLORADO DENVER · PI Edward L Melanson · 1995 to 2026
$32.6M
Decidual nutrient sensing and IGFBP-1 phosphorylation in placental insufficiencyR01HD089980 · NICHD · UNIVERSITY OF COLORADO DENVER · PI GUPTA, MADHULIKA, JANSSON, THOMAS · 2017 to 2021
$1.4M
NICHD NIH HHS R01 HD089980NIDDK NIH HHS P30 DK048520
6 · The paper itself

Abstract

Phosphorylation of decidual IGFBP-1 enhances binding of IGF-I, limiting the bioavailability of this growth factor which may contribute to reduced placental and fetal growth. The mechanisms regulating decidual IGFBP-1 phosphorylation are incompletely understood. Using decidualized human immortalized endometrial stromal cells we tested the hypothesis that low oxygen tension or reduced leucine availability, believed to be common in placental insufficiency, increase the phosphorylation of decidual IGFBP-1. Multiple reaction monitoring-MS (MRM-MS) was used to quantify IGFBP-1 phosphorylation. MRM-MS validated the novel phosphorylation of IGFBP-1 at Ser58, however this site was unaffected by low oxygen tension/leucine deprivation. In contrast, significantly elevated phosphorylation was detected for pSer119, pSer98/pSer101 and pSer169/pSer174 sites. Immunoblotting and dual-immunofluorescence using phosphosite-specific IGFBP-1 antibodies further demonstrated increased IGFBP-1 phosphorylation in HIESC under both treatments which concomitantly reduced IGF-I bioactivity. These data support the hypothesis that down regulation of IGF-I signaling links decidual IGFBP-1 hyperphosphorylation to restricted fetal growth in placental insufficiency.

Indexed as

Analysis of VarianceCells, CulturedDeciduaFemaleFetal Growth RetardationHumansHypoxiaInsulin-Like Growth Factor Binding Protein 1Insulin-Like Growth Factor ILeucineOxygenPhosphorylationPlacental InsufficiencyPregnancyStromal CellsIGF1 protein, humanIGFBP1 protein, humanInsulin-Like Growth Factor Binding Protein 1Insulin-Like Growth Factor ILeucineOxygenCellular stress-stimuliDual immunofluorescence immunocytochemistryFGRHyperphosphorylationIGF bioavailabilityMRM-MS

Identifiers

PMID28435049
PMCPMC5957756
OpenAlexW2606515107

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.