ArticleExtremophiles : life under extreme conditions2017
Characterization of extracellular membrane vesicles of an Antarctic bacterium, Shewanella livingstonensis Ac10, and their enhanced production by alteration of phospholipid composition.
Article in Extremophiles : life under extreme conditions, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Marine Delivery Vehicles: Molecular Components and Applications of Bacterial Extracellular Vesicles.Marine drugs · 2024Review
- Metabolic adaptations ofFrontiers in microbiology · 2024Article
- Environmental and Taxonomic Drivers of Bacterial Extracellular Vesicle Production in Marine Ecosystems.Applied and environmental microbiology · 2023Article
- Eco-evolutionary feedbacks mediated by bacterial membrane vesicles.FEMS microbiology reviews · 2021Review
- Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium,Frontiers in microbiology · 2021Article
- Outer Membrane Vesicles of Gram-Negative Bacteria: An Outlook on Biogenesis.Frontiers in microbiology · 2021Review
- A Novel Lysophosphatidic Acid Acyltransferase ofBiomolecules · 2020Article
- Isolation of a Novel Bacterial Strain Capable of Producing Abundant Extracellular Membrane Vesicles Carrying a Single Major Cargo Protein and Analysis of Its Transport Mechanism.Frontiers in microbiology · 2019Article
- Cracking Open Bacterial Membrane Vesicles.Frontiers in microbiology · 2019Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cold-adapted bacterium, Shewanella livingstonensis Ac10, which produces eicosapentaenoic acid (EPA) as a component of its membrane phospholipids, is useful as a model to study the function of EPA and as a host for heterologous production of thermolabile proteins at low temperatures. In this study, we characterized extracellular membrane vesicles (EMVs) of this bacterium to examine the involvement of EPA in the biogenesis of EMVs and for the future application of EMVs to extracellular protein production. We found that this strain produced EMVs from the cell surface. Cryo-electron microscopic observation showed that the majority of the EMVs had a single-bilayer structure with an average diameter of 110 nm, though EMVs with double-bilayer membranes and other diverse structures were also observed. Quantitative analysis demonstrated that the EMV production was significantly increased (3-5 fold) by the depletion of EPA-containing phospholipids. The lack of EPA also altered the protein composition of EMVs. In particular, incorporation of one of the cold-inducible outer membrane proteins, OmpC176, was significantly increased in EMVs after the depletion of EPA. These results provide a basis for the construction of an EMV-based, low-temperature protein production system and show the involvement of EPA in the regulation of EMV biogenesis.
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