ArticleScientific reports2017
Structure-function analysis of the DNA-binding domain of a transmembrane transcriptional activator.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
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- Nrf1 acts as a highly-conserved determinon for maintaining robust redox homeostasis in the eco-evo-devo process of life histories.Cell stress · 2025Review
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- The Interplay of AphB and CadC to Activate Acid Resistance ofJournal of bacteriology · 2023Article
- Molecular determinants for differential activation of the bile acid receptor from the pathogen Vibrio parahaemolyticus.The Journal of biological chemistry · 2023Article
- Structural Insights into Regulation of Vibrio Virulence Gene Networks.Advances in experimental medicine and biology · 2023Article
- Enhancing the tropism of bacteria via genetically programmed biosensors.Nature biomedical engineering · 2022Article
- Disulfide-compatible phage-assisted continuous evolution in the periplasmic space.Nature communications · 2021Article
- Acid resistance system CadBA is implicated in acid tolerance and biofilm formation and is identified as a new virulence factor of Edwardsiella tarda.Veterinary research · 2021Article
- Programmable receptors enable bacterial biosensors to detect pathological biomarkers in clinical samples.Nature communications · 2021Article
- Proline codon pair selection determines ribosome pausing strength and translation efficiency in bacteria.Communications biology · 2021Article
- Dynamics of chromosomal target search by a membrane-integrated one-component receptor.PLoS computational biology · 2021Article
- The enigma of environmental pH sensing in plants.Nature plants · 2021Review
- How Enzymes, Proteins, and Antibodies Recognize Extended DNAs; General Regularities.International journal of molecular sciences · 2021Review
- Predicting drug targets by homology modelling of Pseudomonas aeruginosa proteins of unknown function.PloS one · 2021Article
- A disulfide constrains the ToxR periplasmic domain structure, altering its interactions with ToxS and bile-salts.Scientific reports · 2020Article
- Switching the Post-translational Modification of Translation Elongation Factor EF-P.Frontiers in microbiology · 2019Article
- DNA-binding directs the localization of a membrane-integrated receptor of the ToxR family.Communications biology · 2019Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transmembrane DNA-binding protein CadC of E. coli, a representative of the ToxR-like receptor family, combines input and effector domains for signal sensing and transcriptional activation, respectively, in a single protein, thus representing one of the simplest signalling systems. At acidic pH in a lysine-rich environment, CadC activates the transcription of the cadBA operon through recruitment of the RNA polymerase (RNAP) to the two cadBA promoter sites, Cad1 and Cad2, which are directly bound by CadC. However, the molecular details for its interaction with DNA have remained elusive. Here, we present the crystal structure of the CadC DNA-binding domain (DBD) and show that it adopts a winged helix-turn-helix fold. The interaction with the cadBA promoter site Cad1 is studied by using nuclear magnetic resonance (NMR) spectroscopy, biophysical methods and functional assays and reveals a preference for AT-rich regions. By mutational analysis we identify amino acids within the CadC DBD that are crucial for DNA-binding and functional activity. Experimentally derived structural models of the CadC-DNA complex indicate that the CadC DBD employs mainly non-sequence-specific over a few specific contacts. Our data provide molecular insights into the CadC-DNA interaction and suggest how CadC dimerization may provide high-affinity binding to the Cad1 promoter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.