Evidence map›Paper›PMID 28431571›Full record

ArticleVirology journal2017

Unique LCR variations among lineages of HPV16, 18 and 45 isolates from women with normal cervical cytology in Ghana.

Adolf K Awua, Richard M K Adanu, Edwin K Wiredu, Edwin A Afari, Vanessa A Zubuch, Richard H Asmah, Alberto Severini

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Article in Virology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
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8citing papers in PubMed, 1 pooled it
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Adolf K AwuaDepartment of Epidemiology and Disease Control, School of Public Health, University of Ghana, Accra, Ghana. a_awua@yahoo.com.ORCID 0000-0003-2516-4514
Richard M K AdanuPopulation, Family and Reproductive Health, School of Public Health, University of Ghana, Accra, Ghana.
Edwin K WireduDepartment of Pathology, School of Biomedical and Allied Health Science, University of Ghana, Korle-Bu, Accra, Ghana.
Edwin A AfariDepartment of Epidemiology and Disease Control, School of Public Health, University of Ghana, Accra, Ghana.
Vanessa A ZubuchNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.
Richard H AsmahDepartment of Medical Laboratory Sciences, School of Biomedical and Allied Health Science, University of Ghana, Korle-Bu, Accra, Ghana.
Alberto SeveriniNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn addition to being useful for classification, sequence variations of human Papillomavirus (HPV) genotypes have been implicated in differential oncogenic potential and a differential association with the different histological forms of invasive cervical cancer. These associations have also been indicated for HPV genotype lineages and sub-lineages. In order to better understand the potential implications of lineage variation in the occurrence of cervical cancers in Ghana, we studied the lineages of the three most prevalent HPV genotypes among women with normal cytology as baseline to further studies.

methodsOf previously collected self- and health personnel-collected cervical specimen, 54, which were positive for HPV16, 18 and 45, were selected and the long control region (LCR) of each HPV genotype was separately amplified by a nested PCR. DNA sequences of 41 isolates obtained with the forward and reverse primers by Sanger sequencing were analysed.

resultsNucleotide sequence variations of the HPV16 genotypes were observed at 30 positions within the LCR (7460 - 7840). Of these, 19 were the known variations for the lineages B and C (African lineages), while the other 11 positions had variations unique to the HPV16 isolates of this study. For the HPV18 isolates, the variations were at 35 positions, 22 of which were known variations of Africa lineages and the other 13 were unique variations observed for the isolates obtained in this study (at positions 7799 and 7813). HPV45 isolates had variations at 35 positions and 2 (positions 7114 and 97) were unique to the isolates of this study.

conclusionThis study provides the first data on the lineages of HPV 16, 18 and 45 isolates from Ghana. Although the study did not obtain full genome sequence data for a comprehensive comparison with known lineages, these genotypes were predominately of the Africa lineages and had some unique sequence variations at positions that suggest potential oncogenic implications. These data will be useful for comparison with lineages of these genotypes from women with cervical lesion and all the forms of invasive cervical cancers.

Indexed as

Genetic VariationGenotypeRegulatory Sequences, Nucleic AcidDNA, ViralFemaleGhanaHumansPapillomaviridaePapillomavirus InfectionsSequence Analysis, DNADNA, ViralAfrican LineageGhanaHPV LineageHPV VariantHuman PapillomavirusLong Control Region

Identifiers

PMID28431571
PMCPMC5401561

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