ArticleMolecular microbiology2017
Staphylococcus aureus nitric oxide synthase (saNOS) modulates aerobic respiratory metabolism and cell physiology.
Article in Molecular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Sustained nitric oxide production by engineered E. coli remodels the tumor microenvironment and potentiates immunotherapy.Nature biotechnology · 2026Article
- The role of nitric oxide synthase in biofilm formation by Staphylococcus aureus.NPJ biofilms and microbiomes · 2025Article
- Altered quorum sensing and physiology of Staphylococcus aureus during spaceflight detected by multi-omics data analysis.NPJ microgravity · 2024Article
- Factors impacting the regulation ofMicrobiology spectrum · 2023Article
- Transcription tuned by S-nitrosylation underlies a mechanism for Staphylococcus aureus to circumvent vancomycin killing.Nature communications · 2023Article
- The Evolution of Nitric Oxide Function: From Reactivity in the Prebiotic Earth to Examples of Biological Roles and Therapeutic Applications.Antioxidants (Basel, Switzerland) · 2022Review
- The nuclear receptor ERR cooperates with the cardiogenic factor GATA4 to orchestrate cardiomyocyte maturation.Nature communications · 2022Article
- Skin-to-blood pH shift triggers metabolome and proteome global remodelling inFrontiers in microbiology · 2022Article
- Involvement of Small Colony Variant-Related Heme Biosynthesis Genes inFrontiers in microbiology · 2021Article
- Quantifying Staphylococcus aureus Membrane Potential Using Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Bacterial-like nitric oxide synthase in the haloalkaliphilic archaeon Natronomonas pharaonis.MicrobiologyOpen · 2020Article
- Exogenous Fatty Acids Remodel Staphylococcus aureus Lipid Composition through Fatty Acid Kinase.Journal of bacteriology · 2020Article
- A Critical Role for Estrogen-Related Receptor Signaling in Cardiac Maturation.Circulation research · 2020Article
- Reactive nitrogen species in host-bacterial interactions.Current opinion in immunology · 2019Review
- Respiration and Small Colony Variants ofMicrobiology spectrum · 2019Review
- The Response ofJournal of bacteriology · 2019Article
- Interplay of Nitric Oxide Synthase (NOS) and SrrAB in Modulation ofInfection and immunity · 2019Article
- Redirection of Metabolism in Response to Fatty Acid Kinase in Staphylococcus aureus.Journal of bacteriology · 2018Article
- A singular nitric oxide synthase with a globin domain found in Synechococcus PCC 7335 mobilizes N from arginine to nitrate.Scientific reports · 2018Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Nitric oxide (NO) is generated from arginine and oxygen via NO synthase (NOS). Staphylococcus aureus NOS (saNOS) has previously been shown to affect virulence and resistance to exogenous oxidative stress, yet the exact mechanism is unknown. Herein, a previously undescribed role of saNOS in S. aureus aerobic physiology was reported. Specifically, aerobic S. aureus nos mutant cultures presented with elevated endogenous reactive oxygen species (ROS) and superoxide levels, as well as increased membrane potential, increased respiratory dehydrogenase activity and slightly elevated oxygen consumption. Elevated ROS levels in the nos mutant likely resulted from altered respiratory function, as inhibition of NADH dehydrogenase brought ROS levels back to wild-type levels. These results indicate that, in addition to its recently reported role in regulating the switch to nitrate-based respiration during low-oxygen growth, saNOS also plays a modulatory role during aerobic respiration. Multiple transcriptional changes were also observed in the nos mutant, including elevated expression of genes associated with oxidative/nitrosative stress, anaerobic respiration and lactate metabolism. Targeted metabolomics revealed decreased cellular lactate levels, and altered levels of TCA cycle intermediates, the latter of which may be related to decreased aconitase activity. Collectively, these findings demonstrate a key contribution of saNOS to S. aureus aerobic respiratory metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.