ArticleBiology of reproduction2017
IFT25, an intraflagellar transporter protein dispensable for ciliogenesis in somatic cells, is essential for sperm flagella formation.
Article in Biology of reproduction, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 64 citations in OpenAlex.
- Identification and Functional Analysis of miRNAs in the Cauda Epididymis of Yak and Cattle.Animals : an open access journal from MDPI · 2026Article
- Male infertility.Systems biology in reproductive medicine · 2025Review
- ARL13B-Cerulean rescues Arl13b-null mouse from embryonic lethality and reveals a role for ARL13B in spermatogenesis.Journal of cell science · 2025Article
- ARL13B-Cerulean rescuesbioRxiv : the preprint server for biology · 2025Article
- Quantification of transcript isoforms at the single-cell level using SCALPEL.Nature communications · 2025Article
- Role of intraflagellar transport protein IFT140 in the formation and function of motile cilia in mammals.Cellular and molecular life sciences : CMLS · 2025Article
- Two-directional trafficking of the IFT25 protein in the developing mouse sperm flagella.Biology of reproduction · 2025Article
- Intraflagellar Transport (IFT) and Sperm Formation.Advances in experimental medicine and biology · 2025Review
- HomozygousZoological research · 2024Article
- CCDC157 is essential for sperm differentiation and shows oligoasthenoteratozoospermia-related mutations in men.Journal of cellular and molecular medicine · 2024Article
- The role of primary cilia in the testis.Current opinion in endocrine and metabolic research · 2024Article
- CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility.Development (Cambridge, England) · 2023Article
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- Cargo adapters expand the transport range of intraflagellar transport.Journal of cell science · 2022Review
- Taurine and its transporter TAUT positively affect male reproduction and early embryo development.Human reproduction (Oxford, England) · 2022Article
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- AXDND1, a novel testis-enriched gene, is required for spermiogenesis and male fertility.Cell death discovery · 2021Article
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- Article
- Some thoughts about intraflagellar transport in reproduction.Molecular reproduction and development · 2021Article
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Authors and funding
15 authors at 8 institutions in 2 countries.
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Abstract
Intraflagellar transport (IFT) is a conserved mechanism essential for the assembly and maintenance of most eukaryotic cilia and flagella. However, IFT25, a component of the IFT complex, is not required for the formation of cilia in somatic tissues. In mice, the gene is highly expressed in the testis, and its expression is upregulated during the final phase when sperm flagella are formed. To investigate the role of IFT25 in sperm flagella formation, the gene was specifically disrupted in male germ cells. All homozygous knockout mice survived to adulthood and did not show any gross abnormalities. However, all homozygous knockout males were completely infertile. Sperm numbers were reduced and these sperm were completely immotile. Multiple morphological abnormalities were observed in sperm, including round heads, short and bent tails, with some tails showing branched flagella and others with frequent abnormal thicknesses, as well as swollen tips of the tail. Transmission electron microscopy revealed that flagellar accessory structures, including the fibrous sheath and outer dense fibers, were disorganized, and most sperm had also lost the "9+2" microtubule structure. In the testis, IFT25 forms a complex with other IFT proteins. In Ift25 knockout testes, IFT27, an IFT25 binding partner, was missing, and IFT20 and IFT81 levels were also reduced. Our findings suggest that IFT25, although not necessary for the formation of cilia in somatic cells, is indispensable for sperm flagellum formation and male fertility in mice.
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