ArticleScientific reports2017
Selection of relatively exact reference genes for gene expression studies in goosegrass (Eleusine indica) under herbicide stress.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 56 citations in OpenAlex.
- Identification and validation of reference genes for quantitative real-time PCR in Corydalis saxicola under various abiotic stresses.Scientific reports · 2026Article
- Subgenomic Distribution and Herbicide Cross-Resistance ofPlant direct · 2026Article
- Non-target-site resistance mechanisms to glyphosate inFrontiers in plant science · 2026Article
- Unraveling the Role of P450 Reductase in Herbicide Metabolic Resistance Mechanism.Plant direct · 2025Article
- Effects of Biological Nitrogen Metabolism on Glufosinate-Susceptible and -Resistant Goosegrass (International journal of molecular sciences · 2023Article
- Potential Role of EPSPS Mutations in the Resistance ofInternational journal of molecular sciences · 2023Article
- Article
- Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.).Scientific reports · 2022Article
- A naturally evolved mutation (Ser59Gly) in glutamine synthetase confers glufosinate resistance in plants.Journal of experimental botany · 2022Article
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- Overexpression of EiKCS confers paraquat-resistance in rice (Oryza sativa L.) by promoting the polyamine pathway.Pest management science · 2022Article
- The analysis of reference genes expression stability in susceptible and resistant Apera spica-venti populations under herbicide treatment.Scientific reports · 2021Article
- Selection and Validation of Reference Genes for RT-qPCR Analysis inInternational journal of molecular sciences · 2021Article
- Validation of Reference Genes for Quantitative PCR in Johnsongrass (Plants (Basel, Switzerland) · 2021Article
- Identification of the most suitable reference gene for gene expression studies with development and abiotic stress response in Bromus sterilis.Scientific reports · 2021Article
- Selection and Validation of Reference Genes for qRT-PCR Analysis in the Oil-Rich Tuber Crop Tiger Nut (International journal of molecular sciences · 2021Article
- Vegetative and Fecundity Fitness Benefit Found in a Glyphosate-ResistantFrontiers in plant science · 2021Article
- Identification and evaluation of suitable reference genes for gene expression analysis in rubber tree leaf.Molecular biology reports · 2020Article
- Identification and evaluation of reference genes for quantitative real-time PCR analysis in Polygonum cuspidatum based on transcriptome data.BMC plant biology · 2019Article
- Selection of Suitable Reference Genes for RT-qPCR Gene Expression Analysis in Siberian Wild Rye (Genes · 2019Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Goosegrass (Eleusine indica) is one of the most serious annual grassy weeds worldwide, and its evolved herbicide-resistant populations are more difficult to control. Quantitative real-time PCR (qPCR) is a common technique for investigating the resistance mechanism; however, there is as yet no report on the systematic selection of stable reference genes for goosegrass. This study proposed to test the expression stability of 9 candidate reference genes in goosegrass in different tissues and developmental stages and under stress from three types of herbicide. The results show that for different developmental stages and organs (control), eukaryotic initiation factor 4 A (eIF-4) is the most stable reference gene. Chloroplast acetolactate synthase (ALS) is the most stable reference gene under glyphosate stress. Under glufosinate stress, eIF-4 is the best reference gene. Ubiquitin-conjugating enzyme (UCE) is the most stable reference gene under quizalofop-p-ethyl stress. The gene eIF-4 is the recommended reference gene for goosegrass under the stress of all three herbicides. Moreover, pairwise analysis showed that seven reference genes were sufficient to normalize the gene expression data under three herbicides treatment. This study provides a list of reliable reference genes for transcript normalization in goosegrass, which will facilitate resistance mechanism studies in this weed species.
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